Microalgae are used as a third-generation biofuel feedstock to overcome the problems, of first- and second-generation feedstocks, such as food versus fuel competition and longer harvesting time. However, the use of microalgae as a feedstock for biodiesel production is limited by the lipid content in the biomass. The development of strategies to enhance both the biomass and lipid content would lead to low-cost processes for biofuel production. In the present study, NaNO₃, K₂HPO₄, NaCl, and initial medium pH were optimized to enhance both biomass yield and lipid content of freshwater microalgae Scenedesmus obliquus NCIM 5586 in a 5-L open pond system under light and dark period using central composite design. A multiobjective optimization method is employed to find the tradeoff between biomass and lipid accumulation and to predict the optimum composition for the multiple responses. The maximized yield of biomass and lipid was observed when concentrations of NaNO₃, K₂HPO_4, and NaCl were 1.60 μM, 178.38 μM, and 24.80 mM, respectively, with initial medium pH of 7.23. The final yields of biomass and lipid at optimized conditions were recorded to be 1275.8 mg/L/day and 50.66% dcw, respectively. The obtained results indicate the significance of Scenedesmus obliquus cultivation in the open pond for the generation of feedstock for 3G biofuels.

微藻被用作第三代生物燃料原料，以克服第一代和第二代原料的问题，例如食物与燃料的竞争以及更长的收获时间。然而，将微藻用作生产生物柴油的原料受到生物质中脂质含量的限制。发展同时增加生物质和脂质含量的策略将导致生物燃料生产的低成本过程。在本研究中，优化了NaNO ₃，K ₂ HPO ₄，NaCl和初始培养基pH值，以提高淡水微藻斜生藻的生物量产量和脂质含量NCIM 5586采用中央复合设计，在明暗时段的5升开放式池塘系统中。多目标优化方法用于找到生物量和脂质积累之间的权衡，并预测多种响应的最佳组成。当NaNO ₃，K ₂ HPO ₄和NaCl的浓度分别为1.60μM，178.38μM和24.80 mM，初始培养基pH为7.23时，观察到最大的生物量和脂质产量。在优化条件下，最终生物量和脂质的最终产量分别记录为1275.8 mg / L / day和50.66％dcw。获得的结果表明，在露天池塘中斜斜栅藻的种植对于产生3G生物燃料的原料具有重要意义。