In this study, we aimed to investigate the role of miRNA-429 in the pathogenesis of pancreatic ductal adenocarcinoma(PDAC) and the potential mechanism of this procedure. Totally 95 consecutive patients with PDAC diagnosed by pathology were retrospectively collected from June 1st , 2015 to August 30th, 2019 in Department of General Surgery, Jingmen First People's Hospital. The human pancreatic cancer cell line Bxpc-3 and Panc-1 were used and the cell proliferation and migration were detected by MTT assays and Transwell assays, respectively. The quantitative real-time polymerase chain reaction (qRT-PCR) was applied to evaluate the expression in RNA level. Our results showed that overexpression of miRNA-429 could suppress the cell invasion, proliferation and metastasis through regulating the process of EMT in PDAC cell line, while low expression of miRNA-429 had the opposite effects. We demonstrated that miRNA-429 had critical roles in the pathogenesis of PDAC. Clinically, we observed that tumor tissues from patients with PDAC exhibited significantly decreasing in miRNA-429 expression compared with the non-tumor tissues. Additionally, decreased expression of miRNA-429 in tumor tissues of patients with PDAC was associated with poorer prognosis and several clinical-pathological characteristics. In conclusion, miRNA-429 exerted anti-tumor functions in PDAC through the regulation of EMT process. The results of this study would provide a novel insight into tumorigenesis and the basis for the development of miRNA-targeting therapies against PDAC.

中文翻译：

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MicroRNA-429在胰腺导管腺癌（PDAC）中上皮向间质转化（EMT）。

在这项研究中，我们旨在研究miRNA-429在胰腺导管腺癌（PDAC）发病机理中的作用以及该程序的潜在机制。2015年6月1日至2019年8月30日在荆门市第一人民医院普外科回顾性收集了95例经病理学诊断的PDAC患者。使用人胰腺癌细胞系Bxpc-3和Panc-1，分别通过MTT测定法和Transwell测定法检测细胞增殖和迁移。应用定量实时聚合酶链反应（qRT-PCR）评估RNA水平的表达。我们的结果表明，miRNA-429的过表达可通过调节PDAC细胞系中EMT的过程来抑制细胞的侵袭，增殖和转移，而低表达的miRNA-429具有相反的作用。我们证明了miRNA-429在PDAC的发病机理中具有关键作用。在临床上，我们观察到与非肿瘤组织相比，患有PDAC的患者的肿瘤组织在miRNA-429表达方面显着降低。此外，miRNA-429在PDAC患者肿瘤组织中的表达降低与预后差和一些临床病理特征有关。总之，miRNA-429通过调控EMT过程在PDAC中发挥抗肿瘤功能。这项研究的结果将为肿瘤发生提供新的见解，并为针对PDAC的miRNA靶向疗法的发展奠定基础。我们观察到，与非肿瘤组织相比，患有PDAC的患者的肿瘤组织显示miRNA-429表达显着降低。此外，miRNA-429在PDAC患者肿瘤组织中的表达降低与预后差和一些临床病理特征有关。总之，miRNA-429通过调控EMT过程在PDAC中发挥抗肿瘤功能。这项研究的结果将为肿瘤发生提供新的见解，并为针对PDAC的miRNA靶向疗法的发展奠定基础。我们观察到，与非肿瘤组织相比，患有PDAC的患者的肿瘤组织显示miRNA-429表达显着降低。此外，miRNA-429在PDAC患者肿瘤组织中的表达降低与预后差和一些临床病理特征有关。总之，miRNA-429通过调控EMT过程在PDAC中发挥抗肿瘤功能。这项研究的结果将为肿瘤发生提供新的见解，并为针对PDAC的miRNA靶向疗法的发展奠定基础。PDAC患者肿瘤组织中miRNA-429表达的降低与预后差和一些临床病理特征有关。总之，miRNA-429通过调控EMT过程在PDAC中发挥抗肿瘤功能。这项研究的结果将为肿瘤发生提供新的见解，并为针对PDAC的miRNA靶向疗法的发展奠定基础。PDAC患者肿瘤组织中miRNA-429表达的降低与预后差和一些临床病理特征有关。总之，miRNA-429通过调控EMT过程在PDAC中发挥抗肿瘤功能。这项研究的结果将为肿瘤发生提供新的见解，并为针对PDAC的miRNA靶向疗法的发展奠定基础。