当前位置:
X-MOL 学术
›
Appl. Spectrosc.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
EXPRESS: Raman and Fluorescence Profiles Modifications of Muscular and Adipose Tissues Exposed to Low Energy X-ray Beams
Applied Spectroscopy ( IF 3.5 ) Pub Date : 2021-02-01 , DOI: 10.1177/0003702821989773 Noemy R Santos 1 , Roseli Künzel 1 , Marcelo B Freitas 2 , Ronaldo S Levenhagen 1 , Ana Paula de A Marques 3 , Lilia C Courrol 1
Applied Spectroscopy ( IF 3.5 ) Pub Date : 2021-02-01 , DOI: 10.1177/0003702821989773 Noemy R Santos 1 , Roseli Künzel 1 , Marcelo B Freitas 2 , Ronaldo S Levenhagen 1 , Ana Paula de A Marques 3 , Lilia C Courrol 1
Affiliation
This work aims to investigate changes induced by low energy radiation in adipose and muscular tissues employing autofluorescence and Raman spectroscopic techniques. X-rays beams expositions with 25 and 35 kV at 0.11, 1.1 and 2.1 Gy radiation dose levels were applied. Changes in Raman line intensities at specific bands assigned to collagen, proteins and lipids were observed. Autofluorescent analysis exhibit variations in the collagen and NAD(P)H emission, resulting from the structural modifications, variations on the reduced/oxidized fluorophores equilibrium followed by radiation exposure. Results show that Raman and fluorescence spectroscopy are suitable techniques to evaluate radiation effects on biomolecules even at low radiation doses and energies.
中文翻译:
EXPRESS:暴露于低能 X 射线束的肌肉和脂肪组织的拉曼和荧光曲线修饰
这项工作旨在利用自发荧光和拉曼光谱技术研究低能辐射在脂肪和肌肉组织中引起的变化。施加 25 和 35 kV、0.11、1.1 和 2.1 Gy 辐射剂量水平的 X 射线束曝光。观察到指定给胶原蛋白、蛋白质和脂质的特定条带的拉曼线强度的变化。自发荧光分析显示出胶原蛋白和 NAD(P)H 发射的变化,这是由于结构修改、还原/氧化荧光团平衡的变化以及随后的辐射暴露所致。结果表明,即使在低辐射剂量和能量下,拉曼光谱和荧光光谱也是评估辐射对生物分子影响的合适技术。
更新日期:2021-02-01
中文翻译:
EXPRESS:暴露于低能 X 射线束的肌肉和脂肪组织的拉曼和荧光曲线修饰
这项工作旨在利用自发荧光和拉曼光谱技术研究低能辐射在脂肪和肌肉组织中引起的变化。施加 25 和 35 kV、0.11、1.1 和 2.1 Gy 辐射剂量水平的 X 射线束曝光。观察到指定给胶原蛋白、蛋白质和脂质的特定条带的拉曼线强度的变化。自发荧光分析显示出胶原蛋白和 NAD(P)H 发射的变化,这是由于结构修改、还原/氧化荧光团平衡的变化以及随后的辐射暴露所致。结果表明,即使在低辐射剂量和能量下,拉曼光谱和荧光光谱也是评估辐射对生物分子影响的合适技术。