In this study, a multistep matrix solid-phase dispersion (MSPD) combining with a high-performance liquid chromatography method was developed for assaying 3 components of different polarities (mannitol, adenosine, and ergosterol) from mushroom samples. MSPD extraction was carried out using 1 g octadecyl-bonded silica as the sorbent material, 9% methanol, 20% methanol, and 100% methanol as eluting solvents for the elution of mannitol, adenosine, and ergosterol, respectively. Mannitol was separated on an NH2P-50 4E column and detected using an evaporative light scattering detector. Adenosine and ergosterol were separated on a Poroshell 120 SB-C18 column and measured at 260 nm and 283 nm, respectively. The developed method showed good linearity (R ≥ 0.9986) within the test range. The relative SD (RSD) of precisions were less than 1.4%, and the recoveries were 95.6%-97.0% (RSD ≤3.0%). Compared with the reported methods, the developed procedure could rapidly prepare components with different polarities (mannitol, adenosine, and ergosterol) from medical mushroom samples with less organic solvent and sample. The method is rapid and eco-friendly, which is helpful to improve the quality evaluation of medicinal mushrooms.

在这项研究中，开发了一种结合高效液相色谱方法的多步基质固相分散体（MSPD），用于测定蘑菇样品中3种极性不同的组分（甘露醇，腺苷和麦角固醇）。MSPD萃取使用1 g十八烷基键合硅胶作为吸附材料，9％甲醇，20％甲醇和100％甲醇作为洗脱溶剂分别洗脱甘露醇，腺苷和麦角固醇。甘露醇在NH2P-50 4E色谱柱上分离，并使用蒸发光散射检测器进行检测。在Poroshell 120 SB-C18色谱柱上分离腺苷和麦角固醇，分别在260 nm和283 nm处测量。所开发的方法具有良好的线性（R≥0.9986）。精密度的相对标准偏差（RSD）小于1.4％，回收率为95.6％-97.0％（RSD≤3.0％）。与已报道的方法相比，该方法可从有机蘑菇和有机溶剂较少的蘑菇样品中快速制备具有不同极性的成分（甘露醇，腺苷和麦角固醇）。该方法快速，环境友好，有助于提高药用蘑菇的质量评价。