Fusicoccin (FC) is a well-known phytotoxin able to induce in Acer pseudoplatanus L. (sycamore) cultured cells, a set of responses similar to those induced by stress conditions. In this work, the possible involvement of peroxynitrite (ONOO⁻) in FC-induced stress responses was studied measuring both in the presence and in the absence of 2,6,8-trihydroxypurine (urate), a specific ONOO⁻ scavenger: (1) cell death; (2) specific DNA fragmentation; (3) lipid peroxidation; (4) production of RNS and ROS; (5) activity of caspase-3-like proteases; and (6) release of cytochrome c from mitochondria, variations in the levels of molecular chaperones Hsp90 in the mitochondria and Hsp70 BiP in the endoplasmic reticulum (ER), and of regulatory 14-3-3 proteins in the cytosol. The obtained results indicate a role for ONOO⁻ in the FC-induced responses. In particular, ONOO⁻ seems involved in a PCD form showing apoptotic features such as specific DNA fragmentation, caspase-3-like protease activity, and cytochrome c release from mitochondria.

中文翻译：

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过氧亚硝酸盐在镰刀菌素诱导的植物培养细胞应答中的可能作用

Fusicoccin（FC）是一种众所周知的植物毒素，能够在Acer pseudoplatanus L.（美国梧桐）培养的细胞中诱导出一系列类似于应激条件下的反应。在这项工作中，过氧亚硝酸盐（ONOO可能参与^- ）在FC-诱导的应激反应进行了研究在存在和不存在2,6,8-三羟基（尿酸盐），特定ONOO的测量^-清除剂：（1 ）细胞死亡；（2）特异性DNA片段化；（3）脂质过氧化；（4）生产RNS和ROS；（5）胱天蛋白酶3样蛋白酶的活性；（6）释放细胞色素c线粒体中分子伴侣Hsp90水平和内质网（ER）中Hsp70 BiP水平的变化以及胞质溶胶中14-3-3调节蛋白的水平变化。得到的结果表明了ONOO一个角色^-在FC诱导的反应。特别是，ONOO ^-似乎参与了PCD形式表示细胞凋亡的功能，如特定的DNA片段化，caspase-3的样蛋白酶活性，细胞色素c ^从线粒体释放。