In the present work, we developed a miniaturized method for determining amylopectin chain length distribution (CLD) by fluorescence-assisted capillary electrophoresis (FACE). The method relies on single granule entrapping into capillaries followed by direct starch gelatinization and amylopectin debranching on carbograph-based solid phase extraction (SPE) cartridges. Sample desalting on HypersepTM tips following APTS-labelling and the use of nanovials allowed for the fluorescence analysis of weakly diluted samples. Consequently, method sensitivity was improved by 500-fold which is compatible with the analysis of single potato starch granules. The method was implemented to determine CLD profiles of single starch granules ranging from 50 to 100 µm in diameter. In these experiments, the relative proportion of starch glucans of up to 30 degrees of polymerization (DP) could be quantified.

中文翻译：

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荧光辅助毛细管电泳（FACE）直至单淀粉颗粒的支链淀粉链长分布的微型化分析

在目前的工作中，我们开发了一种通过荧光辅助毛细管电泳（FACE）测定支链淀粉链长分布（CLD）的小型化方法。该方法依赖于将单个颗粒包裹在毛细管中，然后在基于碳谱仪的固相萃取（SPE）柱上直接进行淀粉糊化和支链淀粉脱支。APTS标记后，在HypersepTM吸头上进行样品脱盐，并使用纳米瓶进行弱稀释样品的荧光分析。因此，方法灵敏度提高了500倍，与单一马铃薯淀粉颗粒的分析兼容。该方法用于确定直径在50至100 µm之间的单个淀粉颗粒的CLD分布图。在这些实验中