Abstract

Background

To investigate the effects of bimatoprost, latanoprost and travoprost on angiogenesis in the chick chorioallantoic membrane (CAM) model in ovo

Materials and methods

Fifty fertilized specific-pathogen-free chick eggs were used in this preclinical, prospective, experimental embryo study. Eggs were randomly distributed into 5 groups of ten eggs. Eggs were placed in the incubator after disinfection of their shells with alcohol and monitored appropriate temperature and humidity. On the 3rd day of incubation, a small window was opened on the eggshell. Bimatoprost in group 1, latanoprost in group 2, travoprost in group 3, bevacizumab in group 4, phosphate-buffered-saline (PBS) used in group 5 was applied by injection to CAM. The sterile film was glued onto the broken part of the shell and the eggs were placed in the incubator again. On the 8th day of incubation, eggs were opened and vascular structures on CAMs were examined. Digital photographs were taken, analysed in the ImageJ open source image processing software and differences between groups were evaluated. Thereafter, VEGF (Vascular endothelial growth factor) levels were measured appropriately in the embryo samples.

Results

All embryos in the prostaglandin groups and the PBS control group were observed to have life signs confirmed by heart rate. In 8 embryos in the bevacizumab group, no life signs were confirmed, while 2 embryos with life signs showed severe hypoplasia. Vascular density, number of vessels and VEGF levels in the bimatoprost, latanoprost and travoprost groups, there were statistically significantly higher than the PBS control group.

Conclusion

This study demonstrates that topical prostaglandin drops increase angiogenesis in the chick CAM model in ovo.

中文翻译：

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外用前列腺素滴剂对蛋内鸡绒毛尿囊膜模型血管生成的影响

摘要

背景

研究比马前列素、拉坦前列素和曲伏前列素对蛋内鸡绒毛尿囊膜 (CAM) 模型血管生成的影响

材料和方法

在这项临床前、前瞻性、实验性胚胎研究中使用了 50 个受精的无特定病原体的鸡卵。鸡蛋随机分成5组，每组10个。用酒精对蛋壳进行消毒后，将蛋放入孵化器中，并监测适当的温度和湿度。孵化第3天，蛋壳上开了一个小窗。第1组的比马前列素、第2组的拉坦前列素、第3组的曲伏前列素、第4组的贝伐单抗、第5组的磷酸盐缓冲液(PBS)通过注射施用于CAM。将无菌膜粘在壳的破损部分上，然后将鸡蛋再次放入孵化器中。在孵化的第 8 天，卵被打开并检查 CAM 上的血管结构。拍摄了数码照片，在 ImageJ 开源图像处理软件中进行分析，并评估组间的差异。此后，在胚胎样品中适当地测量了 VEGF（血管内皮生长因子）水平。

结果

观察到前列腺素组和PBS对照组中的所有胚胎都有心率证实的生命体征。贝伐单抗组8个胚胎未确认生命体征，而有生命体征的2个胚胎出现严重发育不全。比马前列素组、拉坦前列素组和曲伏前列素组的血管密度、血管数和VEGF水平均显着高于PBS对照组。

结论

该研究表明，局部前列腺素滴剂可增加蛋内小鸡 CAM 模型中的血管生成。