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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations
eLife ( IF 7.7 ) Pub Date : 2021-01-19 , DOI: 10.7554/elife.63910
Milo S Johnson 1, 2, 3 , Shreyas Gopalakrishnan 1, 2, 3, 4 , Juhee Goyal 1, 5 , Megan E Dillingham 2, 6 , Christopher W Bakerlee 1, 2, 3, 4 , Parris T Humphrey 1, 2, 3 , Tanush Jagdish 1, 2, 3, 6 , Elizabeth R Jerison 1, 7, 8 , Katya Kosheleva 1, 7 , Katherine R Lawrence 1, 2, 3, 9 , Jiseon Min 1, 2, 3, 4, 5 , Alief Moulana 1 , Angela M Phillips 1 , Julia C Piper 1, 10 , Ramya Purkanti 1, 11 , Artur Rego-Costa 1 , Michael J McDonald 1, 12 , Alex N Nguyen Ba 1, 2, 3, 7, 13 , Michael M Desai 1, 2, 3, 7
Affiliation  

Laboratory experimental evolution provides a window into the details of the evolutionary process. To investigate the consequences of long-term adaptation, we evolved 205 Saccharomyces cerevisiae populations (124 haploid and 81 diploid) for ~10,000,000 generations in three environments. We measured the dynamics of fitness changes over time, finding repeatable patterns of declining adaptability. Sequencing revealed that this phenotypic adaptation is coupled with a steady accumulation of mutations, widespread genetic parallelism, and historical contingency. In contrast to long-term evolution in E. coli, we do not observe long-term coexistence or populations with highly elevated mutation rates. We find that evolution in diploid populations involves both fixation of heterozygous mutations and frequent loss-of-heterozygosity events. Together, these results help distinguish aspects of evolutionary dynamics that are likely to be general features of adaptation across many systems from those that are specific to individual organisms and environmental conditions.

中文翻译:

实验室萌芽酵母种群中 10,000 代的表型和分子进化

实验室实验进化提供了一个了解进化过程细节的窗口。为了研究长期适应的后果,我们在三个环境中进化了 205 个酿酒酵母种群(124 个单倍体和 81 个二倍体),持续了约 10,000,000 代。我们测量了适应性随时间变化的动态,发现了适应性下降的可重复模式。测序表明,这种表型适应与突变的稳定积累、广泛的遗传平行性和历史偶然性相结合。与大肠杆菌的长期进化相比,我们没有观察到长期共存或突变率高度升高的种群。我们发现二倍体种群的进化涉及杂合突变的固定和频繁的杂合性丢失事件。一起,
更新日期:2021-01-19
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