In this study, phenolic composition and in vitro biological activities of ethyl acetate (EAE) and methanol (ME) extracts obtained from the aerial parts of endemic Tanacetum erzincanense were investigated. Total phenolic and flavonoid content of the extracts were determined by Folin-Ciocalteu and Aluminium Chloride colorimetric methods, respectively. Antioxidant capacity of the extracts was evaluated over radical scavenging (DPPH and ABTS) and metal ion reducing power (FRAP and CUPRAC) tests. Individual phenolic compounds in ME was analyzed by High-Performance Liquid Chromatography coupled to Electrospray Ionization Quadropole Time-of-Flight Mass Spectrometry (LC-ESI-QTOF/MS). Cell inhibitory potential of the extracts was tested against colorectal adenocarcinoma (HT-29), breast adenocarcinoma (MCF-7), and hepatocarcinoma (HepG2) cells by 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay. The results showed that ME contains higher TPC (64.4 mgGAE/g) and TFC (62.2 mgQE/g) than those of EAE (41.5 mgGAE/g and 40.0 mgQE/g). LC-ESI-QTOF/MS analysis revealed that ME is rich in phenolic compounds namely, chlorogenic acid, apigenin, quercetin, luteolin, and diosmetin. Antioxidant assay results indicated that ME possess stronger activity than EAE and a power that competes with synthetic antioxidants. XTT assay results demonstrated that although both extracts displayed a considerable cytotoxicity against the tested cancer cell lines in a time and dose-dependent manner, ME expressed its selective inhibitory action towards MCF-7 cells with an IC 50 value of 20.4 µg/mL for 72 h. These results may serve as a basis for further in vivo studies to examine the potential applications of T. erzincanense in food and pharmaceutical industries.

中文翻译：

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LC-ESI-QTOF-MS 测定地方性紫檀提取物的体外抗氧化和细胞毒活性以及酚类含量

在这项研究中，研究了从地方性紫荆的地上部分获得的乙酸乙酯 (EAE) 和甲醇 (ME) 提取物的酚类组成和体外生物活性。提取物的总酚类和类黄酮含量分别通过 Folin-Ciocalteu 和氯化铝比色法测定。通过自由基清除（DPPH 和 ABTS）和金属离子还原能力（FRAP 和 CUPRAC）测试评估提取物的抗氧化能力。ME 中的单个酚类化合物通过高效液相色谱与电喷雾电离四极杆飞行时间质谱 (LC-ESI-QTOF/MS) 联用进行分析。通过 2 次测试提取物对结直肠腺癌 (HT-29)、乳腺癌 (MCF-7) 和肝癌 (HepG2) 细胞的细胞抑制潜力，3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) 测定。结果表明，与 EAE（41.5 mgGAE/g 和 40.0 mgQE/g）相比，ME 含有更高的 TPC（64.4 mgGAE/g）和 TFC（62.2 mgQE/g）。LC-ESI-QTOF/MS 分析表明，ME 富含酚类化合物，即绿原酸、芹菜素、槲皮素、木犀草素和地奥司美丁。抗氧化分析结果表明，ME 具有比 EAE 更强的活性和与合成抗氧化剂竞争的能力。XTT 测定结果表明，尽管两种提取物均以时间和剂量依赖性方式对所测试的癌细胞系显示出相当大的细胞毒性，但 ME 对 MCF-7 细胞表现出选择性抑制作用，IC 50 值为 20.4 µg/mL，持续 72 H。