This manuscript outlines the kinetics of two main repair pathways of DNA double-strand break (DSB) in eukaryotes: non-homologous end joining (NHEJ) and homologous recombination repair (HRR). In this review, we discuss the precise study of recruitment kinetics of repair proteins based on the latest technologies in the past two decades. Then we simulate the theoretical description of the DNA repair process by mathematical models. In our study, the consecutive reactions chain (CRC) model and continuous-time random walk (CTRW) model have been unified by us, so that we can obtain the function of the number of intermediates with time in the same framework of equations, overcome the incompatibility between the two models. On this basis, we propose a data fitting workflow using these both models. Finally, we give an overview of different real-time quantitative methods and the new mechanism complexity that can be found from the corresponding dynamic models.

这份手稿概述了真核生物中 DNA 双链断裂 (DSB) 的两种主要修复途径的动力学：非同源末端连接 (NHEJ) 和同源重组修复 (HRR)。在这篇综述中，我们讨论了基于过去二十年最新技术的修复蛋白募集动力学的精确研究。然后我们通过数学模型模拟了DNA修复过程的理论描述。在我们的研究中，我们统一了连续反应链（CRC）模型和连续时间随机游走（CTRW）模型，这样我们就可以在相同的方程框架中获得中间体数量随时间的函数，克服两个模型之间的不兼容。在此基础上，我们提出了使用这两种模型的数据拟合工作流程。最后，