The amount of publicly available DNA sequence data is drastically increasing, making it a tedious task to create sequence databases necessary for the design of diagnostic assays. The selection of appropriate sequences is especially challenging in genes affected by frequent point mutations such as antibiotic resistance genes. To overcome this issue, we have designed the webtool resiDB, a rapid and user-friendly sequence database manager for bacteria, fungi, viruses, protozoa, invertebrates, plants, archaea, environmental and whole genome shotgun sequence data. It automatically identifies and curates sequence clusters to create custom sequence databases based on user-defined input sequences. A collection of helpful visualization tools gives the user the opportunity to easily access, evaluate, edit, and download the newly created database. Consequently, researchers do no longer have to manually manage sequence data retrieval, deal with hardware limitations, and run multiple independent software tools, each having its own requirements, input and output formats. Our tool was developed within the H2020 project FAPIC aiming to develop a single diagnostic assay targeting all sepsis-relevant pathogens and antibiotic resistance mechanisms. ResiDB is freely accessible to all users through https://residb.ait.ac.at/.

可公开获得的DNA序列数据的数量急剧增加，这使得创建诊断分析设计所需的序列数据库成为一项繁琐的任务。在受频繁点突变影响的基因（例如抗生素抗性基因）中，选择合适的序列尤其具有挑战性。为了克服这个问题，我们设计了webtool resiDB，这是一种快速且易于使用的序列数据库管理器，用于细菌，真菌，病毒，原生动物，无脊椎动物，植物，古细菌，环境和全基因组shot弹枪序列数据。它会根据用户定义的输入序列自动识别和管理序列簇以创建自定义序列数据库。一系列有用的可视化工具为用户提供了轻松访问，评估，编辑和下载新创建的数据库的机会。因此，研究人员不再需要手动管理序列数据检索，处理硬件限制并运行多个独立的软件工具，每个工具都有其自己的要求，输入和输出格式。我们的工具是在H2020 FAPIC项目中开发的，旨在开发针对所有败血症相关病原体和抗生素耐药机制的单一诊断测定。所有用户均可通过https://residb.ait.ac.at/免费访问ResiDB。我们的工具是在FAPIC H2020项目中开发的，旨在开发针对所有败血症相关病原体和抗生素耐药性机制的单一诊断测定。所有用户均可通过https://residb.ait.ac.at/免费访问ResiDB。我们的工具是在H2020 FAPIC项目中开发的，旨在开发针对所有败血症相关病原体和抗生素耐药机制的单一诊断测定。所有用户均可通过https://residb.ait.ac.at/免费访问ResiDB。