Thalamic astrocytes and oligodendrocytes are coupled via gap junctions and form panglial networks. Here, we show that these networks have a key role in energy supply of neurons. Filling an astrocyte or an oligodendrocyte in acute slices with glucose or lactate is sufficient to rescue the decline of stimulation-induced field post-synaptic potential (fPSP) amplitudes during extracellular glucose deprivation (EGD). In mice lacking oligodendroglial coupling, loading an astrocyte with glucose does not rescue the EGD-mediated loss of fPSPs. Monocarboxylate and glucose transporters are required for rescuing synaptic activity during EGD. In mice deficient in astrocyte coupling, filling of an oligodendrocyte with glucose does not rescue fPSPs during EGD. Our results demonstrate that, in the thalamus, astrocytes and oligodendrocytes are jointly engaged in delivering energy substrates for sustaining neuronal activity and suggest that oligodendrocytes exert their effect mainly by assisting astrocytes in metabolite transfer to the postsynapse.

丘脑星形胶质细胞和少突胶质细胞通过间隙连接耦合并形成神经网络。在这里，我们表明这些网络在神经元的能量供应中起着关键作用。在急性切片中用葡萄糖或乳酸填充星形胶质细胞或少突胶质细胞足以挽救细胞外葡萄糖剥夺 (EGD) 期间刺激诱导的场突触后电位 (fPSP) 幅度的下降。在缺乏少突胶质细胞偶联的小鼠中，用葡萄糖加载星形胶质细胞并不能挽救 EGD 介导的 fPSP 损失。单羧酸盐和葡萄糖转运蛋白是 EGD 期间拯救突触活动所必需的。在星形胶质细胞偶联缺陷的小鼠中，用葡萄糖填充少突胶质细胞并不能在 EGD 期间拯救 fPSP。我们的研究结果表明，在丘脑中，