当前位置: X-MOL 学术Cell Rep. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
Cell Reports ( IF 8.8 ) Pub Date : 2021-01-19 , DOI: 10.1016/j.celrep.2020.108640
Nitika Badjatia 1 , Matthew J Rossi 1 , Alain R Bataille 1 , Chitvan Mittal 2 , William K M Lai 2 , B Franklin Pugh 2
Affiliation  

In multicellular eukaryotes, RNA polymerase (Pol) II pauses transcription ~30–50 bp after initiation. While the budding yeast Saccharomyces has its transcription mechanisms mostly conserved with other eukaryotes, it appears to lack this fundamental promoter-proximal pausing. However, we now report that nearly all yeast genes, including constitutive and inducible genes, manifest two distinct transcriptional stall sites that are brought on by acute environmental signaling (e.g., peroxide stress). Pol II first stalls at the pre-initiation stage before promoter clearance, but after DNA melting and factor acquisition, and may involve inhibited dephosphorylation. The second stall occurs at the +2 nucleosome. It acquires most, but not all, elongation factor interactions. Its regulation may include Bur1/Spt4/5. Our results suggest that a double Pol II stall is a mechanism to downregulate essentially all genes in concert.



中文翻译:

急性压力通过酵母菌中两个独立的 RNA 聚合酶 II 停滞事件驱动全球抑制

在多细胞真核生物中,RNA 聚合酶 (Pol) II 在启动后暂停转录约 30-50 bp。而出芽酵母酵母其转录机制与其他真核生物大多是保守的,它似乎缺乏这种基本的启动子近端暂停。然而,我们现在报告几乎所有酵母基因,包括组成型和诱导型基因,都表现出两个不同的转录停滞位点,这些位点是由急性环境信号传导(例如,过氧化物应激)引起的。Pol II 首先在启动子清除之前的启动前阶段停止,但在 DNA 熔解和因子获取之后,并且可能涉及抑制的去磷酸化。第二个停顿发生在 +2 核小体。它获得了大多数(但不是全部)伸长因子相互作用。其监管可能包括 Bur1/Spt4/5。我们的研究结果表明,双 Pol II 失速是一种协同下调所有基因的机制。

更新日期:2021-01-19
down
wechat
bug