The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task. The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly. Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading. Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds. Microscopy results showed that the chitosan coating with a thickness of around 10 nm was formed in the bulk of BC, covering each microfibril. It was found that the inner specific surface area increased 1.5 times on deposition of chitosan from the solutions in carbonic acid.

用壳聚糖生物聚合物对细菌纤维素（BC）表面进行功能化以扩展改性BC的可能应用领域是一项重要的科学任务。在生物相容性和生态友好性方面，在这项工作中进行的碳酸溶液中这种复合物的产生具有多个优点。通过tri标记的壳聚糖放射性检测方法对复合物中壳聚糖的含量进行了定量分析，结果表明壳聚糖的负载量增加了三倍。不同的理化方法显示壳聚糖成功掺入BC基质并通过氢键与其相互作用。显微镜检查结果表明，在BC本体中形成了厚度约10 nm的壳聚糖涂层，覆盖了每个微纤维。