The white snook (Centropomus viridis) is a species with great aquaculture potential, therefore, the present study aims two objectives; 1) to characterize digestive enzymatic activity during larval development and; 2) characterize the diversity and functionality of digestive enzymes in an early juvenile stage of C. viridis. On first feeding, larvae were 2.75 ± 0.09 mm total length and grew to 24.35 ± 4.35 mm after 42 days. The specific activity of acid proteases was detected with low activity from day 1 (Days after hatching, DAH) to day 21, and increased at 23 DAH, reaching maximum activity at 40 DAH. Meanwhile, digestive enzymatic activity of alkaline proteases was observed from hatching to day 40. From hatching until 40 DAH, the presence of alkaline proteases, trypsin, chymotrypsin, leucine aminopeptidase, α-amylase and lipase were detected with different activity patterns. Total and specific activity of digestive enzymes on juveniles showed higher activity of acid than alkaline proteases, while α-amylase had a higher activity than lipases. The maximum activity of acid proteases was observed at 40 °C and at pH 2.0, while digestive alkaline proteases showed maximum activity at 60 °C. α-amylases and lipases showed their maximum activities at 50 °C. The maximum alkaline protease and α-amylase activities were found at pH 7 to 9. The enzymatic activity of juvenile's acid proteases was highly inhibited by Pepstatin A (96%), however the activity of alkaline proteases incubated with PHEN, TLCK, SBTI, PMFS, and OVO showed reductions of 51.29, 30.16, 27.98, 27.26, and 24.68% respectively. Lipase activity was totally inhibited with Ebelactone B, and highly inhibited by Ebelactone A, Orlistat, 1% SDS, and PMFS (96.12, 77.16, 64.22, and 51.29%, respectively). Seven bands (102.6, 65.7, 58.9, 40.6, 27.4, 21.5, and 15.8 kDa) resolved by protein electrophoresis were detected with alkaline protease activity, identified as serine proteases and metalloproteases, and three bands (57, 43.9, and 32.6 kDa) with lipase-like activity were also identified. These results show that C. viridis ontogeny entails an indirect development of its digestive system with a functional stomach on day 23, and characterized by increases in pepsin activity. Indeed, white snook ontogeny shows a similar developmental trajectory to other carnivorous marine fish, showing a diversity of alkaline proteases that work in a wide range of pH and temperatures. Thus, the identification of these enzymes provides advantages to formulate white snook aqua-feeds.

白鼻ook（Centropomus viridis）是具有巨大水产养殖潜力的物种，因此，本研究的目标是两个目标；1）表征幼体发育过程中的消化酶活性；2）表征青铜梭菌幼体早期消化酶的多样性和功能。初次喂养时，幼虫的总长度为2.75±0.09 mm，并在42天后长至24.35±4.35 mm。从第1天（孵化后几天，DAH）到第21天，检测到酸性蛋白酶的比活性较低，并在23 DAH时增加，在40 DAH时达到最大活性。同时，从孵化到第40天观察到碱性蛋白酶的消化酶活性。从孵化到40 DAH，碱性蛋白酶，胰蛋白酶，胰凝乳蛋白酶，亮氨酸氨肽酶，α-淀粉酶和脂肪酶的存在以不同的活性模式被检测到。幼体中消化酶的总活性和比活性显示出比碱性蛋白酶更高的酸活性，而α-淀粉酶的活性高于脂肪酶。在40°C和pH 2.0下观察到酸性蛋白酶的最大活性，而消化性碱性蛋白酶在60°C时显示最大活性。α-淀粉酶和脂肪酶在50°C时显示最大活性。碱性蛋白酶和α-淀粉酶的最大活性在pH 7至9时发现。PepstatinA（96％）高度抑制了青少年的酸性蛋白酶的酶活性，但是与PHEN，TLCK，SBTI，PMFS一起孵育的碱性蛋白酶的活性和OVO分别减少了51.29、30.16、27.98、27.26和24.68％。脂肪酶活性被Ebelactone B完全抑制，而被Ebelactone A，Orlistat，1％SDS和PMFS高度抑制（分别为96.12、77.16、64.22和51.29％）。通过蛋白质电泳检测到七个具有碱性蛋白酶活性的条带（102.6、65.7、58.9、40.6、27.4、21.5和15.8 kDa），具有碱性蛋白酶活性，被鉴定为丝氨酸蛋白酶和金属蛋白酶，并确定了三个具有脂肪酶样活性的条带（57、43.9和32.6 kDa）。这些结果表明C. viridis个体发育需要在第23天间接消化具有功能性胃的消化系统，并以胃蛋白酶活性增加为特征。的确，白斯诺克个体发育显示出与其他肉食性海鱼相似的发育轨迹，显示出在各种pH和温度范围内都起作用的多种碱性蛋白酶。因此，这些酶的鉴定为配制白斯诺克水族饲料提供了优势。