Background and Objectives

We demonstrated that the mRNA induction of S100s in rat peripheral leukocytes by severe hyperglycemia was reduced by inhibiting postprandial hyperglycemia. Here, we compared inflammatory gene expression in peripheral leukocytes between type 2 diabetes mellitus (T2DM) patients undergoing dietary therapy alone and healthy volunteers, and between T2DM patients undergoing dietary therapy alone and those undergoing such therapy in combination with drug therapy using the α-glucosidase inhibitor miglitol.

Methods

T2DM patients who had undertaken dietary therapy alone or in combination with drug therapy using miglitol for ≥ 8 weeks and healthy volunteers were subjected to a meal tolerance test and glucose concentration, neutrophil elastase concentration, and mRNA expression analyses of peripheral leukocytes by microarray and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) immediately before and 180 min after a meal.

Results

Blood glucose concentrations 60 min after a meal were lower in T2DM patients with dietary + miglitol therapy than in those with dietary therapy alone. Neutrophil elastase concentrations at 60 and 120 min after a meal were lower in T2DM patients with dietary + miglitol therapy than in those with dietary therapy alone. Expression levels of S100A8 in a fasting state and S100A6, S100A8, and S100A9 180 min after a meal were higher in T2DM patients with dietary therapy alone than in healthy volunteers. Expression levels of S100A12 in a fasting state and 180 min after a meal were higher in T2DM patients with dietary therapy alone than in T2DM patients with dietary + miglitol therapy.

Conclusions

S100 genes were more highly expressed in T2DM patients with dietary therapy than in healthy volunteers.

中文翻译：

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S100基因在饮食治疗的2型糖尿病患者的外周血白细胞中高表达

背景和目标

我们证明，通过抑制餐后高血糖，可减轻严重高血糖在大鼠外周血白细胞中S100的mRNA诱导。在这里，我们比较了单独进行饮食治疗的2型糖尿病（T2DM）患者和健康志愿者以及单独进行饮食治疗的T2DM患者与结合使用α-葡萄糖苷酶药物治疗的T2DM患者之间外周血白细胞的炎症基因表达抑制剂米格列醇。

方法

单独接受饮食治疗或联合使用米格列醇治疗≥8周的药物治疗的T2DM患者和健康志愿者进行餐耐受性测试，血糖浓度，中性粒细胞弹性蛋白酶浓度以及微阵列和定量反向分析外周血白细胞的mRNA表达饭前和饭后180分钟进行转录聚合酶链反应（qRT-PCR）。

结果

饮食+米格列醇治疗的T2DM患者餐后60分钟的血糖浓度低于单纯饮食治疗的T2DM患者。饮食+米格列醇治疗的T2DM患者在餐后60和120分钟时的中性粒细胞弹性蛋白酶浓度比单纯饮食治疗的患者低。的表达水平S100A8在空腹状态，S100A6，S100A8和S100A9饭后180分钟是2型糖尿病患者的饮食治疗比单纯在健康志愿者高。单独饮食治疗的T2DM患者的空腹状态和饭后180分钟时S100A12的表达水平高于饮食饮食+米格列醇治疗的T2DM患者。

结论

与健康志愿者相比，饮食治疗的T2DM患者中S100基因的表达更高。