Grapevine, one of the most important fruit crops worldwide is host to various pathogens, some of which have been spread intercontinentally in the early history of viticulture. One of the most problematic diseases is downy mildew, caused by the oomycete Plasmopara viticola (Berk. & Curt.) Berl. & de Toni which was introduced to Europe from North America in the 19th century. In addition to the establishment of chemical plant protection measures, extensive resistance breeding activities have succeeded in establishing varieties that are able to limit the propagation of this pathogen Recent studies have indicated an important role of subtilisin-like serine proteases (subtilases) in association with this increased disease tolerance. Gene expression analysis of two candidate subtilases was performed in seven cultivars with different levels of susceptibility to P. viticola. Two P. viticola isolates with different degrees of virulence were tested. Our results show that subtilases display different patterns of expression: Cucumisin exhibits peaks of expression, while VviSBT5.3a presents a bimodal behaviour. Nevertheless, both subtilases have a more pronounced response to the more virulent P. viticola isolate, which supports the hypothesis that the two subtilases are involved in the early events of the defense mechanisms.

葡萄是世界上最重要的水果作物之一，是多种病原体的寄主，其中一些病原体已在葡萄栽培的早期历史中广泛传播。问题最严重的疾病之一是霜霉病，它是由卵菌的Plasmopara viticola引起的。（伯克和库特）伯尔。＆de Toni于19世纪从北美引入欧洲。除了建立化学植物保护措施外，广泛的抗性育种活动还成功地建立了能够限制这种病原体繁殖的品种。最近的研究表明，与此相关的枯草杆菌蛋白酶样丝氨酸蛋白酶（枯草蛋白酶）具有重要作用。疾病抵抗力增强。对七个对葡萄假单胞菌敏感性不同的品种进行了两种枯草杆菌酶的基因表达分析。两个P.霜霉病具有不同程度的毒性株进行了测试。我们的结果表明，枯草杆菌蛋白酶表现出不同的表达模式：黄瓜蛋白酶表现出表达峰，而VviSBT5.3a表现出双峰行为。然而，枯草都必须更加剧毒更明显的反应P.霜霉病分离，支持这两个枯草参与防御机制的早期事件的假说。