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Optimization and kinetic modeling of Trametes maxima IIPLC-32 laccase and application in recalcitrant dye decolorization
New Journal of Chemistry ( IF 3.3 ) Pub Date : 2020-12-28 , DOI: 10.1039/d0nj05179a
Sunil Kumar Suman 1, 2, 3, 3, 4 , Manisha Malhotra 1, 2, 3 , Shailendra Singh Khichi 3, 4 , Sanjoy Ghosh 3, 4 , Suman Lata Jain 1, 2, 3
Affiliation  

Fungal laccases catalyze a wide range of reactions that have fostered an escalating demand in diverse sectors. Higher productivity in a short period remains a major challenge that needs to be fulfilled for successful industrial applications. In the present study, statistical optimization methods were utilized for high-yielding laccase production by the fungus Trametes maxima IIPLC-32 that achieved 103 IU mL−1 activity and a 13.3-fold increase compared to that under unoptimized conditions. Optimization using the Plackett–Burman design (PBD) and Box–Behnken design (BBD) identified that glucose, xylidine, veratryl alcohol, CuSO4, and potassium phosphate significantly influenced the laccase production. Furthermore, it also revealed that glucose contributed maximally to the overall laccase production, followed by xylidine and veratryl alcohol. Mathematical models were used for kinetic analysis. Kinetics of biomass formation followed the logistic growth model (with the model parameter values of Xmax = 9.54 g L−1, μmax = 0.77 d−1, and R2 = 0.95). Laccase production kinetics followed the Luedeking–Piret model (with the model parameters of α = 0.12 IU mg−1, β = 1.57 IU mg−1 d−1, and R2 = 0.90), indicating that the laccase produced by Trametes maxima IIPLC-32 is partially growth associated. The logistic mass balance equation adequately described substrate utilization. The model parameters of Yx/s = 0.96 g g−1 and m = 0.05 g g−1 d−1 were retrieved from the logistic mass balance equation. The applicability of the enzyme produced from the optimized process was checked for the decolorization of remazol brilliant blue R, and the maximum decolorization efficiency of 92.3% was achieved within 8 h of reaction without the addition of a mediator.

中文翻译:

Trametes maxima IIPLC-32漆酶的优化和动力学建模及其在难降解染料脱色中的应用

真菌漆酶催化了各种各样的反应,从而促进了各个领域不断增长的需求。在短时间内提高生产率仍然是成功工业应用需要解决的主要挑战。在本研究中,统计优化方法被用于真菌Trametes maxima IIPLC-32的高产漆酶生产,与未优化的条件相比,该酶实现了103 IU mL -1的活性并增加了13.3倍。使用Plackett–Burman设计(PBD)和Box–Behnken设计(BBD)进行的优化确定了葡萄糖,二甲苯胺,藜芦醇,CuSO 4和磷酸钾显着影响漆酶的生产。此外,它还揭示了葡萄糖对整个漆酶生产的贡献最大,其次是二甲苯胺和藜芦醇。数学模型用于动力学分析。的生物质形成动力学遵循物流增长模型(带的模型参数值X最大9.54克L = -1μ最大= 0.77 d -1,和- [R 2 = 0.95)。漆酶产生动力学遵循Luedeking-Piret模型(模型参数为α = 0.12 IU mg -1β = 1.57 IU mg -1 d -1,且R 2 = 0.90),表明Trametes maxima IIPLC-32产生的漆酶部分与生长相关。逻辑质量平衡方程充分描述了底物利用率。从对数质量平衡方程中检索到Y x / s = 0.96 gg -1m = 0.05 gg -1 d -1的模型参数。检查了由优化方法生产的酶的适用性,以分析了间苯二酚艳兰R的脱色,并且在不添加介体的情况下,在反应8小时内达到了92.3%的最大脱色效率。
更新日期:2021-01-18
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