Parkinson’s disease (PD) is a nervous disorder, affects physical movement, and leads to difficulty in balancing, walking, and coordination. A novel sensor is mandatory to determine PD and monitor the progress of the treatment. Neurofilament light chain (NfL) has been recognized as a good biomarker for PD and also helps to distinguish between PD and atypical PD syndromes. Immunosensor was generated by current–volt measurement on gap-fingered interdigitated electrode with silicon dioxide surface to determine NfL level. To enhance the detection, anti-NfL antibody was complexed with gold-nanourchin and immobilized on the sensing electrode. The current–volt response was gradually increased at the linear detection range from 100 fM to 1 nM. Limit of detection and sensitivity were 100 fM with the signal-to-noise ratio at n = 3 on a linear curve (y = 0.081x + 1.593; R ² = 0.9983). Limit of quantification falls at 1 pM and high performance of the sensor was demonstrated by discriminating against other neurogenerative disease markers, in addition, it was reproducible even in serum-spiked samples. This method of detection system aids to measure the level of NfL and leads to determine the condition with PD.

帕金森氏病（PD）是一种神经疾病，会影响身体运动，并导致平衡，行走和协调困难。必须使用新型传感器来确定PD并监测治疗进度。神经丝轻链（NfL）被公认为是PD的良好生物标记，也有助于区分PD和非典型PD综合征。免疫传感器是通过在具有二氧化硅表面的带指叉的叉指电极上进行电流伏特测量而产生的，以确定NfL水平。为了增强检测，将抗NfL抗体与金纳米金复合并固定在传感电极上。在100 fM至1 nM的线性检测范围内，电流-电压响应逐渐增加。检测限和灵敏度为100 fM，信噪比为n线性曲线上的= 3（y = 0.081 x + 1.593；R ² = 0.9983）。定量限在1 pM，通过区分其他神经发生性疾病标志物证明了传感器的高性能，此外，即使在血清标本中也可以重现。这种检测系统的方法有助于测量NfL的水平，并通过PD来确定病情。