当前位置: X-MOL 学术Front. Cell Dev. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
ZP4 Is Present in Murine Zona Pellucida and Is Not Responsible for the Specific Gamete Interaction
Frontiers in Cell and Developmental Biology ( IF 5.5 ) Pub Date : 2020-12-21 , DOI: 10.3389/fcell.2020.626679
Mª José Izquierdo-Rico , Carla Moros-Nicolás , Míriam Pérez-Crespo , Ricardo Laguna-Barraza , Alfonso Gutiérrez-Adán , Frédéric Veyrunes , José Ballesta , Vincent Laudet , Pascale Chevret , Manuel Avilés

Mammalian eggs are surrounded by an extracellular matrix called the zona pellucida (ZP). This envelope participates in processes such as acrosome reaction induction, sperm binding, protection of the oviductal embryo, and may be involved in speciation. In eutherian mammals, this coat is formed of three or four glycoproteins (ZP1–ZP4). While Mus musculus has been used as a model to study the ZP for more than 35 years, surprisingly, it is the only eutherian species in which the ZP is formed of three glycoproteins Zp1, Zp2, and Zp3, Zp4 being a pseudogene. Zp4 was lost in the Mus lineage after it diverged from Rattus, although it is not known when precisely this loss occurred. In this work, the status of Zp4 in several murine rodents was tested by phylogenetic, molecular, and proteomic analyses. Additionally, assays of cross in vitro fertilization between three and four ZP rodents were performed to test the effect of the presence of Zp4 in murine ZP and its possible involvement in reproductive isolation. Our results showed that Zp4 pseudogenization is restricted to the subgenus Mus, which diverged around 6 MYA. Heterologous in vitro fertilization assays demonstrate that a ZP formed of four glycoproteins is not a barrier for the spermatozoa of species with a ZP formed of three glycoproteins. This study identifies the existence of several mouse species with four ZPs that can be considered suitable for use as an experimental animal model to understand the structural and functional roles of the four ZP proteins in other species, including human.



中文翻译:

ZP4存在于小鼠Zona Pellucida中,对特定的配子相互作用不负责

哺乳动物卵被称为透明带(Zona pellucida,ZP)的细胞外基质包围。该包膜参与顶体反应诱导,精子结合,输卵管胚胎保护等过程,并可能参与物种形成。在真热的哺乳动物中,这种皮毛由三种或四种糖蛋白(ZP1-ZP4)形成。而小家鼠 已被用作研究ZP的模型超过35年,令人惊讶的是,它是ZP由三种糖蛋白Zp1,Zp2和Zp3形成的唯一的欧亚物种。 Zp4 是一个假基因。 Zp4 迷失在 小家鼠 它脱离后的血统 ,但尚不清楚何时会确切发生这种损失。在这项工作中,通过系统发育,分子和蛋白质组学分析测试了Zp4在几种鼠类啮齿动物中的状态。另外,交叉分析体外进行了三到四个ZP啮齿动物的受精试验,以测试ZP4在鼠类ZP中的存在及其可能参与生殖分离的作用。我们的结果表明Zp4 伪生成仅限于子属 小家鼠,这大约在6 MYA左右。异源的体外受精试验表明,由四个糖蛋白形成的ZP不会阻碍由三个糖蛋白形成的ZP物种的精子。这项研究确定了几种具有四个ZP的小鼠物种的存在,可以认为它们适合用作实验动物模型,以了解这四个ZP蛋白在其他物种(包括人类)中的结构和功能作用。

更新日期:2021-01-18
down
wechat
bug