Maize by-product from the bioethanol industry (distiller’s dried grains with solubles, DDGS) is a source of ferulated arabinoxylan (AX), which is a health-promoting polysaccharide. In the present study, AX from DDGS was fermented by a representative colonic bacterial mixture (Bifidobacterium longum, Bifidobacterium adolescentis, and Bacteroides ovatus), and the effect of the fermented AX (AX-f) on the proliferation of the cell line Caco-2 was investigated. AX was efficiently metabolized by these bacteria, as evidenced by a decrease in the polysaccharide molecular weight from 209 kDa to < 50 kDa in AX-f, the release of ferulic acid (FA) from polysaccharide chains (1.14 µg/mg AX-f), and the short-chain fatty acids (SCFA) production (277 µmol/50 mg AX). AX-f inhibited the proliferation of Caco-2 cells by 80–40% using concentrations from 125–1000 µg/mL. This dose-dependent inverse effect was attributed to the increased viscosity of the media due to the polysaccharide concentration. The results suggest that the AX-f dose range and the SCFA and free FA production are key determinants of antiproliferative activity. Using the same polysaccharide concentrations, non-fermented AX only inhibited the Caco-2 cells proliferation by 8%. These findings highlight the potential of AX recovered from the maize bioethanol industry as an antiproliferative agent once fermented by colonic bacteria.

中文翻译：

---

从玉米生物乙醇工业中回收的阿魏木聚糖发酵物

来自生物乙醇行业的玉米副产品（蒸馏水含可溶物的干粮，DDGS）是一种阿糖木聚糖（AX）的来源，阿糖木聚糖是一种促进健康的多糖。在本研究中，DDGS的AX通过代表性的结肠细菌混合物（长双歧杆菌，青春双歧杆菌和卵形杆菌）发酵。），并研究了发酵AX（AX-f）对细胞系Caco-2增殖的影响。AX被这些细菌有效地代谢，AX-f中的多糖分子量从209 kDa降低至<50 kDa，多糖链中阿魏酸（FA）的释放（1.14 µg / mg AX-f）证明了这一点。 ，以及短链脂肪酸（SCFA）的产量（277 µmol / 50 mg AX）。使用125-1000 µg / mL的浓度，AX-f可抑制Caco-2细胞的增殖80-40％。这种剂量依赖性的逆作用归因于由于多糖浓度引起的介质粘度的增加。结果表明AX-f剂量范围和SCFA和游离FA的产生是抗增殖活性的关键决定因素。使用相同的多糖浓度 非发酵的AX只抑制Caco-2细胞增殖8％。这些发现凸显了从玉米生物乙醇工业中回收的AX一旦被结肠细菌发酵后作为抗增殖剂的潜力。