Molecular characterization of porcine rotavirus A from India revealing zooanthroponotic transmission,Animal Biotechnology

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Molecular characterization of porcine rotavirus A from India revealing zooanthroponotic transmission
Animal Biotechnology ( IF 3.7 ) Pub Date : 2021-01-18 , DOI: 10.1080/10495398.2020.1868486
Gazanfar Abass ₁ , Zunjar Baburao Dubal ₁ , Kaushal K Rajak ₁ , Balasaheb M Kale ₁ , Abhay Raorane ₁ , Nitin Dudhe ₁ , Bilal Ahmad Malla ₁ , Dhananjay Desai ₁ , Dharmendra K Sinha ₁ , Obli R Vinodh Kumar ₁ , Yashpal Singh Malik ₁

Affiliation

Abstract

Rotaviruses A (RVA) are leading causes of diarrhea and dehydration in piglets and imply great economic loss to the pig farming community. In this study, the porcine RVA genotypes circulating in western and northern parts of India were determined by screening 214 fecal samples from diarrheic (n = 144) and non-diarrheic (n = 70) pigs. Subsequently, the structural (VP4 and VP7) and nonstructural (NSP3, and NSP4) genes were amplified, sequenced, and genetically characterized. The RVA positivity percentage was 7.94% (17/214) by RNA-PAGE and 10.28% (22/214) by RT-PCR. Higher RVA positivity was observed in samples from Uttar Pradesh (24.07%) followed by Maharashtra (6.77%) and Goa (2.38%). The sequence and automated genotyping software analysis confirmed the circulation of G4P[6] and G9P[13] RVA strains in porcine population. To note, the sequence similarity of the VP7 gene of Porcine/INDIA/RVA/PK-13 IVRI/Maharashtra/G4 and Porcine/INDIA/RVA/P-8/IVRI/U.P./G9 strain showed a relationship of 96.83 and 98.89% at the nucleotide level with human RVA strains indicating inter-species transmission. Additionally, the NSP3 (T1) and NSP4 (E1) genes (genotypes) also showed genetic relatedness with human RVA strains. Overall, the nucleotide sequences of VP7, NSP3, and NSP4 genes of porcine RVA indicate zooanthroponotic transmission. Further, we report the detection of G9P[13] RVA strain in porcine for the first time from India.

HIGHLIGHTS
RVA positivity was 7.94% (17/214) by RNA-PAGE and 10.28% (22/214) by RT-PCR
The RVA strain G9P[13] reported for the first time in Indian piglets
VP7, NSP3 and NSP4 genes analysis of porcine RVA showed genetic relatedness with human strains indicating evidence of zooanthroponotic transmission

中文翻译：

来自印度的猪轮状病毒 A 的分子特征揭示了动物源性传播

摘要

轮状病毒 A (RVA) 是仔猪腹泻和脱水的主要原因，给养猪业造成巨大的经济损失。在这项研究中，通过筛选 214 个来自腹泻（n = 144）和非腹泻（n = 70) 猪。随后，对结构（VP4 和 VP7）和非结构（NSP3 和 NSP4）基因进行了扩增、测序和遗传表征。RVA 阳性百分比通过 RNA-PAGE 检测为 7.94% (17/214)，通过 RT-PCR 检测为 10.28% (22/214)。在来自北方邦 (24.07%) 的样本中观察到较高的 RVA 阳性率，其次是马哈拉施特拉邦 (6.77%) 和果阿邦 (2.38%)。序列和自动基因分型软件分析证实了 G4P[6] 和 G9P[13] RVA 菌株在猪群中的循环。需要注意的是，Porcine/INDIA/RVA/PK-13 IVRI/Maharashtra/G4 和 Porcine/INDIA/RVA/P-8/IVRI/UP/G9 菌株的 VP7 基因序列相似性分别为 96.83 和 98.89%在核苷酸水平上与人类 RVA 菌株表明物种间传播。此外，NSP3 (T1) 和 NSP4 (E1) 基因（基因型）也显示出与人类 RVA 菌株的遗传相关性。总体而言，猪 RVA 的 VP7、NSP3 和 NSP4 基因的核苷酸序列表明人畜共生传播。此外，我们报告了首次在印度检测到猪中的 G9P[13] RVA 菌株。