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Transcriptome changes of Takifugu obscurus liver after acute exposure to phenanthrene
Physiological Genomics ( IF 4.6 ) Pub Date : 2021-01-18 , DOI: 10.1152/physiolgenomics.00100.2020
Shu-Lun Jiang 1 , Di-An Fang 2 , Dong-Po Xu 2
Affiliation  

Phenanthrene (Phe) is a model compound in PAHs research. Reportedly, Phe treatment induced oxidative stress and histological disorders to Takifugu obscurus liver. In the present study, to further explore the molecular responses of T. obscurus liver to Phe exposure, transcriptome sequencing was applied to compare mRNA transcription profiles between Phe treatment and the control. Compared with the control, 1581 and 1428 genes were significantly upregulated and downregulated in Phe treatment, respectively. Further analysis revealed that Phe treatment mainly upregulated genes in Ras-MAPK and PI3K-akt signaling pathways, which represented insulin resistance and further activated the FOXO signaling pathway. The triacylglycerol biosynthesis was promoted but the gluconeogenesis process was inhibited in response to Phe treatment, demonstrating that Phe exposure disturbed the sugar and lipid metabolism. Moreover, Phe treatment upregulated the apelin-AJP and ErbB signaling pathways, promoting angiogenesis in T. obscurus liver. Insulin resistance, promoted triacylglycerol biosynthesis and angiogenesis might explain the molecular mechanisms underlying carcinogenic toxicity of Phe. Overall, this study provides new insights to understand the environmental risk of Phe to fishes.

中文翻译:

暗花泷急性暴露于菲后的转录组变化

菲 (Phe) 是多环芳烃研究中的模型化合物。据报道,Phe 治疗诱导了 Takifugu obscurus 肝脏的氧化应激和组织学紊乱。在本研究中,为了进一步探索 T. obscurus 肝脏对 Phe 暴露的分子反应,应用转录组测序来比较 Phe 处理和对照之间的 mRNA 转录谱。与对照相比,1581和1428个基因在Phe处理中分别显着上调和下调。进一步分析发现,Phe处理主要上调了代表胰岛素抵抗的Ras-MAPK和PI3K-akt信号通路中的基因,进一步激活了FOXO信号通路。Phe 处理促进了三酰甘油的生物合成,但抑制了糖异生过程,证明 Phe 暴露扰乱了糖和脂质代谢。此外,Phe 治疗上调 apelin-AJP 和 ErbB 信号通路,促进 T. obscurus 肝脏中的血管生成。胰岛素抵抗、促进三酰基甘油生物合成和血管生成可能解释了 Phe 致癌毒性的分子机制。总的来说,这项研究为了解 Phe 对鱼类的环境风险提供了新的见解。
更新日期:2021-01-18
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