In the present work, we established and characterized a 3D functional polarized primary bovine oviduct epithelial cells (BOECs) culture on free‐floating type I collagen hydrogels (rafts) at an air‐liquid interface (ALI). Intercellular junctions, ultrastructural cellular morphology and the expression of the OVGP1 closely recapitulated those of the in vivo epithelium lining. These morphological and physiological epithelial cell features were maintained under standard DMEM/F12 with 10% foetal bovine serum culture medium for at least 28 days of ALI culture. The versatility of the BOECs raft cultures should allow testing of toxicity compounds, in vitro evaluation of physiological or pathological oviductal states, and the study of epithelial‐mesenchymal interactions that are critical for the maintenance of oviductal homeostasis.

中文翻译：

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牛上皮细胞在胶原蛋白筏上的长期体外培养

在目前的工作中，我们在气液界面 (ALI) 的自由漂浮 I 型胶原水凝胶 (筏) 上建立并表征了 3D 功能极化原代牛输卵管上皮细胞 (BOEC)。细胞间连接、超微结构细胞形态和 OVGP1 的表达紧密地概括了体内上皮衬里的那些。这些形态和生理上皮细胞特征在标准 DMEM/F12 下用 10% 胎牛血清培养基维持至少 28 天的 ALI 培养。BOECs 筏培养的多功能性应允许测试毒性化合物、生理或病理性输卵管状态的体外评估，以及对维持输卵管稳态至关重要的上皮间充质相互作用的研究。