The early diagnosis of recurrence and metastasis is critically important for decreasing the morbidity and mortality associated with oral cancers. Although liquid biopsy methods hold great promise that provide a successive “time‐slice” profile of primary and metastatic oral cancer, the development of non‐invasive, rapid, simple, and cost‐effective liquid biopsy techniques remains challenging. In this study, an ultrasensitive and selective electrochemical liquid biopsy is developed for oral cancer screening based on tracking trace amounts of cancer biomarker by functionalized asymmetric nano‐channels. Detection via antigen–antibody reactions is assayed by evaluating changes in ionic current. Upon the recognition of cancer biomarker antigens in bio‐fluids, the inner wall of nano‐channel immobilized with the corresponding antibodies undergoes molecular conformation transformation and surface physicochemical changes, which significantly regulate the ion transport through the nano‐channel and help achieve sensitivity with a detection limit of 10^–12 g mL⁻¹. Furthermore, owing to the specificity of the monoclonal antibody for the antigen, the nano‐channel exhibits high selectivity for the biomarker than for structurally similar biological molecules present in bio‐fluids. The effectiveness of this technique is confirmed through the diagnosis of clinical cases of oral squamous cell carcinoma. This study presents a novel diagnostic tool for oral cancer detection in bio‐fluids.

中文翻译：

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用于口腔癌筛查的超灵敏和选择性电化学生物流体活检

复发和转移的早期诊断对于降低与口腔癌相关的发病率和死亡率至关重要。尽管液体活检方法具有很大的前景，可以为原发性和转移性口腔癌提供连续的“时间片”剖面，但开发无创、快速、简单且具有成本效益的液体活检技术仍然具有挑战性。在这项研究中，基于功能化不对称纳米通道跟踪痕量癌症生物标志物，开发了一种用于口腔癌筛查的超灵敏和选择性电化学液体活检。通过评估离子电流的变化来分析通过抗原-抗体反应的检测。在识别生物体液中的癌症生物标志物抗原后，^–12克毫升^-1。此外，由于单克隆抗体对抗原的特异性，纳米通道对生物标志物的选择性高于对生物体液中结构相似的生物分子的选择性。该技术的有效性通过口腔鳞状细胞癌临床病例的诊断得到证实。本研究提出了一种用于在生物体液中检测口腔癌的新型诊断工具。