当前位置: X-MOL 学术Primates › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genetic analysis of an insular population of Sapajus nigritus (Primates: Cebidae) in Rio de Janeiro state, Brazil
Primates ( IF 1.7 ) Pub Date : 2021-01-18 , DOI: 10.1007/s10329-020-00880-9
Diego Mattos Penedo 1 , Jorge Luís Azevedo de Armada 2 , Mariela Nieves 3 , Carlos Eduardo da Silva Verona 4 , Andréa Maria de Oliveira 5 , Emidio José de Sousa Dos Santos 6 , Denise Monnerat Nogueira 2
Affiliation  

The black-horned capuchin (Sapajus nigritus) is a neotropical primate with wide distribution from southeastern Brazil to northeastern Argentina. Although this species has been described with coat pattern variation, even with intrapopulational differences, and characterized as having the greatest genetic diversity among Sapajus species, there are still few studies on natural populations that contribute to the knowledge of this intraspecific variability. We examined individuals from an as yet unstudied population of Ilha da Marambaia, Rio de Janeiro (RJ) state, Brazil, compared with published data for S. nigritus. We sought to confirm the species through phenotypic and genetic characterization using C-banding and fluorescence in situ hybridization with #11qHe+/21WCP probes for chromosomal constitutive heterochromatin (He+) patterns, and cytochrome c oxidase I and II gene sequences for phylogenetic analysis. The coat presented two color patterns, varying from brown to blackish on the body, yellow to brown on the chest, and white to yellow on the face, besides the presence and shape of the tufts on the head, corresponding to S. nigritus. He+ was identified in pairs 4, 12, 13 and 17, and less consistently in pairs 6, 19 and 21, already described for this species. While most Sapajus species have a large He+ block, here pair 11 was identified without extracentromeric He+, the same as reported for S. nigritus from Argentina. Molecular analysis showed divergence of this population from other S. nigritus sequences, reinforcing a trend already demonstrated when samples from RJ are compared with the rest of the distribution, which may represent an evolutionary deviation.

中文翻译:

巴西里约热内卢州 Sapajus nigritus(灵长类:Cebidae)岛群的遗传分析

黑角卷尾猴 (Sapajus nigritus) 是一种新热带灵长类动物,广泛分布于巴西东南部至阿根廷东北部。尽管该物种被描述为具有毛皮模式变异,即使存在种群内差异,并且被描述为在 Sapajus 物种中具有最大的遗传多样性,但对有助于了解这种种内变异性的自然种群的研究仍然很少。我们检查了来自巴西里约热内卢 (RJ) 州 Ilha da Marambaia 尚未研究的人群中的个体,并与已发布的黑链球菌数据进行了比较。我们试图通过使用 C 带和荧光原位杂交与 #11qHe+/21WCP 探针进行染色体组成型异染色质 (He+) 模式的表型和遗传表征来确认物种,用于系统发育分析的细胞色素 c 氧化酶 I 和 II 基因序列。被毛呈现出两种颜色的图案,从身体的棕色到黑色,胸部的黄色到棕色,脸上的白色到黄色,此外头部有簇绒的存在和形状,对应于S. nigritus。He+ 在第 4、12、13 和 17 对中被鉴定,在第 6、19 和 21 对中不太一致,已经对该物种进行了描述。虽然大多数 Sapajus 物种有一个大的 He+ 块,但这里的第 11 对被鉴定为没有着丝粒外的 He+,与来自阿根廷的 S. nigritus 报告的相同。分子分析表明,该种群与其他黑斑海棠序列存在差异,加强了将来自 RJ 的样本与分布的其余部分进行比较时已经证明的趋势,这可能代表了进化偏差。
更新日期:2021-01-18
down
wechat
bug