Staphylococcus aureus (S.aureus) is both a colonizer as well as a human pathogen that causes a variety of diseases. Mupirocin is a topical antimicrobial agent which is very effective against S.aureus infection. However, treating the S.aureus infection using mupirocin could be complicated due to biofilm formation. Consequently, resistance to mupirocin occurs and leads to chronic infection. The combination of mupirocin with a compound that has biofilm eradicating effect would be an ideal solution for effectively treating biofilm infections. Therefore, in this study, we have investigated the biofilm inhibitory and eradication effect of mupirocin with three essential oils (Cinnamon Oil (CO), Eugenol (EU) and Eucalyptus Oil (EO)) against sessile S.aureus. From these preliminary results, it was found that the mupirocin-CO (0.2 µg/ml-5.218 mg/ml) combination has a better synergistic antibiofilm effect against sessile S.aureus and the fractional inhibitory concentration index was found to be 0.458. The best combination of mupirocin with CO was loaded into a non-greasy O/W cream. The physico-chemical and microbiological evaluations were carried out for the prepared cream. The prepared cream has better biofilm eradication activity (40%) when compared to a marketed cream (20%).

中文翻译：

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用于根除生物膜介导的抗菌素耐药性的莫匹罗星和精油乳膏的配方开发

金黄色葡萄球菌 (S.aureus) 既是定殖者，也是导致多种疾病的人类病原体。莫匹罗星是一种局部抗菌剂，对金黄色葡萄球菌感染非常有效。然而，由于生物膜的形成，使用莫匹罗星治疗金黄色葡萄球菌感染可能很复杂。因此，发生对莫匹罗星的抗性并导致慢性感染。莫匹罗星与具有生物膜清除作用的化合物的组合将是有效治疗生物膜感染的理想解决方案。因此，在本研究中，我们研究了莫匹罗星与三种精油（肉桂油 (CO)、丁香酚 (EU) 和桉树油 (EO)）对无柄金黄色葡萄球菌的生物膜抑制和根除作用。根据这些初步结果，发现莫匹罗星-CO (0.2 µg/ml-5. 218 mg/ml) 组合对固着金黄色葡萄球菌具有更好的协同抗生物膜作用，并且发现分数抑制浓度指数为 0.458。将莫匹罗星与 CO 的最佳组合装入不油腻的 O/W 乳膏中。对制备的乳膏进行物理化学和微生物学评价。与市售乳膏 (20%) 相比，制备的乳膏具有更好的生物膜根除活性 (40%)。