In this work, seven indigenous macrofungal isolates were selected to screen for their laccase production capability. Among them, isolates viz., Pleurotus eryngii, Pleurotus florida, Pleurotus sajor caju and Gandoderma lucidum were found to exhibit high laccase activity in the preliminary studies and were thus selected for the optimization studies with an aim to enhance laccase production. The pH optimization studies were carried out between pH range of 4–6. The laccase activity and biomass were found to be optimum at pH 4, 4.5, 4.5 and 5 for P. eryngii, P. florida, P. sajor caju and G. lucidum, respectively. Optimization studies with chemical inducers namely, tannic acid, 2,6 dimethoxyphenol and copper sulphate at three different concentration levels were conducted and tannic acid at 2 mM concentration was found to increase the laccase activity to about 45% followed by 2,6 dimethoxyphenol (2 mM) with an increase of about 43% and copper sulphate (0.1 mM) showing 21% increase in the yield. Biodegradation studies utilizing laccase isolated from P. eryngii, P. florida and P. sajor caju was carried out for a commonly detected fluoroquinolone antibiotic, levofloxacin, in water and pharmaceutical wastewater. The results indicated that the degradation efficiency of levofloxacin using laccase isolated from P. eryngii (88.9%) was comparable to commercial laccase (89%). When the cost economics of using crude laccase was evaluated against commercial laccase it was evident that the total cost of the treatment could be reduced by 71.7% if commercial grade laccase was replaced by crude enzyme extracted from indigenous macrofungi such Pleurotus eryngii, Pleurotus florida, and Pleurotus sajor caju indicating a promising and cost-effective alternative for wastewater treatment.

在这项工作中，选择了七种本地大型真菌分离物来筛选它们的漆酶生产能力。其中，在初步研究中发现分离株即杏鲍菇、Pleurotus florida、Pleurotus sajor caju和Gandoderma lucidum表现出高漆酶活性，因此被选择用于优化研究，以提高漆酶产量。pH 优化研究在 4-6 的 pH 范围内进行。发现对于杏鲍菇、佛罗里达鲍鱼、金桔和灵芝，漆酶活性和生物量分别在4、4.5、4.5和 5 时是最佳的。使用化学诱导剂即单宁酸、2,6 二甲氧基苯酚和硫酸铜在三种不同浓度水平进行优化研究，发现 2 mM 浓度的单宁酸将漆酶活性提高到约 45%，然后是 2,6 二甲氧基苯酚（2 mM) 增加约 43%，硫酸铜 (0.1 mM) 的产量增加 21%。对水和制药废水中常见的氟喹诺酮类抗生素左氧氟沙星进行了生物降解研究，该研究利用从P. eryngii、P. florida和P. sajor caju中分离出来的漆酶进行了生物降解研究。结果表明，用杏鲍菇漆酶降解左氧氟沙星的效率较高。(88.9%) 与商业漆酶 (89%) 相当。当使用粗制漆酶的成本经济性与商业漆酶进行评估时，很明显，如果商业级漆酶被从本地大型真菌如杏鲍菇、佛罗里达侧耳和Pleurotus sajor caju表明了一种有前途且具有成本效益的废水处理替代方案。