Stigma color plays an important role in pollination. In nature, melon (Cucumis melo L.) stigmas are either yellow or green; however, a review of the literature found no report on how stigma color affects pollination and fruit development in melon. Here, we used an F₂ melon population derived from a cross between ‘MR-1’ (P₁, with green stigmas) and ‘M1–32’ (P₂, with yellow stigmas), and performed genetic analysis and mapping. The results of bulked segregant analysis allowed the identification of genetic loci controlling stigma color on chromosomes 6 and 8. An F₂ population consisting of 150 individuals was used for initial mapping. A genetic map of 304.17 cM was constructed using 37 cleaved amplified polymorphism sequence (CAPS) markers. We identified one major quantitative trait locus (QTL) and one minor QTL for stigma color. The major QTL GS8.1 was further mapped to a 4.13 cM interval between CAPS markers 8C-10 and 8C-16, which explained 27.04% of the phenotypic variation. In addition, GS6.1 was mapped between E-49 and 6A-7, explaining 18.6% of the phenotypic variation. This study provides a theoretical basis for the fine mapping and cloning of melon genes controlling stigma color.

柱头颜色在授粉中起着重要作用。在自然界中，甜瓜（Cucumis melo L.）柱头呈黄色或绿色；然而，对文献的回顾没有发现柱头颜色如何影响甜瓜的授粉和果实发育。在这里，我们使用了来自'MR-1'（P ₁，带有绿色柱头）和'M1-32'（P ₂，带有黄色柱头）之间的杂交的F ₂甜瓜种群，并进行了遗传分析和定位。批量分离分析的结果允许鉴定控制染色体 6 和 8 上柱头颜色的遗传位点。 F ₂由 150 个个体组成的种群用于初始映射。使用 37 个切割的扩增多态性序列 (CAPS) 标记构建了 304.17 cM 的遗传图谱。我们为柱头颜色确定了一个主要数量性状基因座 (QTL) 和一个次要 QTL。主要 QTL GS8.1进一步定位到 CAPS 标记 8C-10 和 8C-16 之间的 4.13 cM 间隔，这解释了 27.04% 的表型变异。此外，GS6.1位于 E-49 和 6A-7 之间，解释了 18.6% 的表型变异。该研究为甜瓜控制柱头颜色基因的精细定位和克隆提供了理论依据。