We report a facile single-step synthesis approach for the development of reduced graphene oxide (rGO) supported copper nanoparticles, which can also perform as a peroxidase enzyme mimicking catalyst. The entire synthesis procedure uses a single reducing agent to reduce graphene oxide and the copper precursor at a relatively lower temperature of 70 °C to generate the Cu-rGO composite, where copper nanoparticles are homogenously decorated on the rGO surface. Morphological and structural analysis shows that the crinkled structure of the rGO protects the copper nanoparticles from excessive oxidation and results in the development of a stable Cu-rGO composite. The as-prepared Cu-rGO composite was studied for its efficacy to perform as a peroxidase mimic by catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), a peroxidase substrate in the presence of hydrogen peroxide. Cu-rGO was successfully able to catalyze hydrogen peroxide and oxidation reaction of TMB followed by a color change. Our results indicate that the Cu-rGO composite has a largely unexplored potential for application in the development of hybrid sensing and detection systems for hydrogen peroxide, which can also be applied for glucose detection.

我们报告了一种简便的一步合成方法，用于开发还原型氧化石墨烯（rGO）负载的铜纳米颗粒，它还可以作为过氧化物酶的模拟催化剂。整个合成过程使用一种还原剂在相对较低的70°C温度下还原氧化石墨烯和铜前体 °C生成Cu-rGO复合材料，其中铜纳米颗粒均匀地装饰在rGO表面上。形态和结构分析表明，rGO的褶皱结构可保护铜纳米颗粒免于过度氧化，并导致开发出稳定的Cu-rGO复合材料。研究了所制备的Cu-rGO复合材料在过氧化氢存在下通过催化3,3'，5,5'-四甲基联苯胺（TMB）（一种过氧化物酶底物）的氧化来充当过氧化物酶模拟物的功效。Cu-rGO成功地能够催化过氧化氢和TMB的氧化反应，然后发生颜色变化。我们的结果表明，Cu-rGO复合材料在开发过氧化氢混合感测和检测系统中具有广阔的应用潜力，