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Expression analysis of chick Frizzled receptors during spinal cord development
Gene Expression Patterns ( IF 1.2 ) Pub Date : 2021-01-16 , DOI: 10.1016/j.gep.2021.119167
Abdulmajeed Fahad Alrefaei 1 , Andrea E Münsterberg 2 , Grant N Wheeler 2
Affiliation  

Frizzleds (Fzds) are transmembrane receptors that can transduce signals dependent upon binding of Wnts, a large family of secreted glycoproteins homologous to the Drosophila wingless gene. FZDs are critical for a wide variety of normal and pathological developmental processes. In the nervous system, Wnts and Frizzleds play an important role in anterior-posterior patterning, cell fate decisions, proliferation, and synaptogenesis. Here, we preformed a comprehensive expression profile of Wnt receptors (FZD) by using situ hybridization to identify FZDs that are expressed in dorsal-ventral regions of the neural tube development. Our data show specific expression for FZD1,2,3,7,9 and 10 in the chick developing spinal cord. This expression profile of cFZD receptors offers the basis for functional studies in the future to determine roles for the different FZD receptors and their interactions with Wnts during dorsal-ventral neural tube development in vivo. Furthermore, we also show that co-overexpression of Wnt1/3a by in vivo electroporation affects FZD7/10 expression in the neural tube. This illustrates an example of Wnts-FZDs interactions during spinal cord neurogenesis.



中文翻译:

小鸡卷曲受体在脊髓发育过程中的表达分析

Frizzleds (Fzds) 是一种跨膜受体,可以根据 Wnts 的结合来转导信号,Wnts 是与果蝇无翅基因同源的一大类分泌糖蛋白。FZD 对各种正常和病理发育过程至关重要。在神经系统中,Wnts 和 Frizzleds 在前后模式、细胞命运决定、增殖和突触发生中发挥重要作用。在这里,我们通过使用原位杂交来识别在神经管发育的背腹区域表达的 FZD,从而形成了 Wnt 受体 (FZD) 的综合表达谱。我们的数据显示 FZD1、2、3、7、9 和 10 在发育中的小鸡脊髓中的特异性表达。体内。此外,我们还表明 Wnt1/3a 通过体内电穿孔的共过表达影响神经管中的 FZD7/10 表达。这说明了脊髓神经发生过程中 Wnts-FZDs 相互作用的一个例子。

更新日期:2021-01-24
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