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Ethanol production process driving changes on industrial strains
FEMS Yeast Research ( IF 3.2 ) Pub Date : 2021-01-08 , DOI: 10.1093/femsyr/foaa071 Sheila Tiemi Nagamatsu 1, 2 , Natalia Coutouné 3 , Juliana José 2 , Mateus Bernabe Fiamenghi 2 , Gonçalo Amarante Guimarães Pereira 2 , Juliana Velasco de Castro Oliveira 3 , Marcelo Falsarella Carazzolle 2
中文翻译:
乙醇生产工艺驱动工业菌株变化
更新日期:2021-02-18
FEMS Yeast Research ( IF 3.2 ) Pub Date : 2021-01-08 , DOI: 10.1093/femsyr/foaa071 Sheila Tiemi Nagamatsu 1, 2 , Natalia Coutouné 3 , Juliana José 2 , Mateus Bernabe Fiamenghi 2 , Gonçalo Amarante Guimarães Pereira 2 , Juliana Velasco de Castro Oliveira 3 , Marcelo Falsarella Carazzolle 2
Affiliation
ABSTRACT
Ethanol production has key differences between the two largest producing countries of this biofuel, Brazil and the USA, such as feedstock source, sugar concentration and ethanol titers in industrial fermentation. Therefore, it is highly probable that these specificities have led to genome adaptation of the Saccharomyces cerevisiae strains employed in each process to tolerate different environments. In order to identify particular adaptations, in this work, we have compared the genomes of industrial yeast strains widely used to produce ethanol from sugarcane, corn and sweet sorghum, and also two laboratory strains as reference. The genes were predicted and then 4524 single-copy orthologous were selected to build the phylogenetic tree. We found that the geographic location and industrial process were shown as the main evolutionary drivers: for sugarcane fermentation, positive selection was identified for metal homeostasis and stress response genes, whereas genes involved in membrane modeling have been connected with corn fermentation. In addition, the corn specialized strain Ethanol Red showed an increased number of copies of MAL31, a gene encoding a maltose transporter. In summary, our work can help to guide new strain chassis selection for engineering strategies, to produce more robust strains for biofuel production and other industrial applications.
中文翻译:
乙醇生产工艺驱动工业菌株变化
摘要
乙醇生产在这种生物燃料的两个最大生产国巴西和美国之间存在主要差异,例如工业发酵中的原料来源、糖浓度和乙醇滴度。因此,这些特异性极有可能导致酿酒酵母的基因组适应在每个过程中使用的菌株以耐受不同的环境。为了确定特定的适应性,在这项工作中,我们比较了广泛用于从甘蔗、玉米和甜高粱生产乙醇的工业酵母菌株的基因组,以及作为参考的两个实验室菌株。对基因进行预测,然后选择4524个单拷贝直向同源物构建系统发育树。我们发现地理位置和工业过程显示为主要的进化驱动因素:对于甘蔗发酵,金属稳态和应激反应基因被确定为正选择,而涉及膜建模的基因与玉米发酵有关。此外,玉米特化菌株乙醇红的 MAL31 拷贝数增加,MAL31 是一种编码麦芽糖转运蛋白的基因。