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iosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring
Sensors ( IF 3.9 ) Pub Date : 2021-01-16 , DOI: 10.3390/s21020602
Sandra Leonardo , Anna Toldrà , Mònica Campàs

The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand, biosensors based on isothermal DNA amplification methods have emerged, which avoid the need for thermal cycling, thus facilitating their integration into small and low-cost devices for in situ monitoring. This review focuses on the breakthroughs made on biosensors based on isothermal amplification methods for the detection of bacteria in the field of food safety and environmental monitoring. Optical and electrochemical biosensors based on loop mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), helicase dependent amplification (HDA), strand displacement amplification (SDA), and isothermal strand displacement polymerisation (ISDPR) are described, and an overview of their current advantages and limitations is provided. Although further efforts are required to harness the potential of these emerging analytical techniques, the coalescence of the different isothermal amplification techniques with the wide variety of biosensing detection strategies provides multiple possibilities for the efficient detection of bacteria far beyond the laboratory bench.

中文翻译:

基于等温DNA扩增的生物传感器在食品安全和环境监测中的细菌检测

细菌污染的简便快速扩散及其对人体健康的危害,显然需要一种替代常规费时的实验室细菌检测技术的分析方法。为了满足这一需求,已经出现了基于等温DNA扩增方法的生物传感器,该传感器避免了热循环的需要,从而有利于将其集成到用于现场监测的小型低成本设备中。这篇综述集中在基于等温扩增方法的生物传感器在食品安全和环境监测领域中对细菌检测的突破。基于环介导的等温扩增(LAMP),滚环扩增(RCA),重组酶聚合酶扩增(RPA)的光学和电化学生物传感器 描述了解旋酶依赖性扩增(HDA),链置换扩增(SDA)和等温链置换聚合(ISDPR),并概述了它们目前的优势和局限性。尽管需要进一步努力来利用这些新兴的分析技术的潜力,但是不同等温扩增技术与多种生物传感检测策略的结合为远远超出实验室工作范围的细菌有效检测提供了多种可能性。
更新日期:2021-01-18
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