Several studies were devoted to the design of molecularly imprinted polymer (MIP)-based sensors for the detection of a given protein. Here, we bring elements that could contribute to the understanding of the interaction mechanism involved in the recognition of a protein by an imprint. For this purpose, a polydopamine (PDA)-MIP was designed for bovine serum albumin (BSA) recognition. Prior to BSA grafting, the gold surfaces were functionalized with mixed self-assembled monolayers of (MUDA)/(MHOH) (1/9, v/v). The MIP was then elaborated by dopamine electropolymerization and further extraction of BSA templates by incubating the electrode in proteinase K solution. Three complementary techniques, electrochemistry, zetametry, and Fourier-transform infrared spectrometry, were used to investigate pH and ionic strength effects on a MIP’s design and the further recognition process of the analytes by the imprints. Several MIPs were thus designed in acidic, neutral, and basic media and at various ionic strength values. Results indicate that the most appropriate conditions, to achieve a successful MIPs, were an ionic strength of 167 mM and a pH of 7.4. Sensitivity and dissociation constant of the designed sensor were of order of (3.36 ± 0.13) µA·cm⁻²·mg⁻¹·mL and (8.56 ± 6.09) × 10⁻¹¹ mg/mL, respectively.

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离子强度和pH值影响研究

多项研究致力于设计基于分子印迹聚合物（MIP）的传感器，用于检测给定的蛋白质。在这里，我们介绍了可能有助于理解与印迹识别蛋白质有关的相互作用机制的元素。为此，设计了一种聚多巴胺（PDA）-MIP用于牛血清白蛋白（BSA）识别。在BSA接枝之前，金表面用（MUDA）/（MHOH）（1/9，v / v）的混合自组装单层功能化）。然后通过多巴胺电聚合和通过在蛋白酶K溶液中孵育电极进一步提取BSA模板来完善MIP。三种互补技术，即电化学，折光法和傅里叶变换红外光谱法，用于研究pH值和离子强度对MIP设计的影响以及通过压印进一步识别分析物的过程。因此，在酸性，中性和碱性介质中设计了几种MIP，并采用了各种离子强度值。结果表明，实现成功的MIP的最合适条件是离子强度为167 mM，pH值为7.4。设计传感器的灵敏度和解离常数分别为（3.36±0.13）µA·cm ^-2 ·mg ^-1 ·mL和（8.56±6.09）×10 ^-11 分别为mg / mL。