当前位置: X-MOL 学术Plants › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Morphological and Molecular Characterization of Pratylenchus dakotaensis n. sp. (Nematoda: Pratylenchidae), a New Root-Lesion Nematode Species on Soybean in North Dakota, USA
Plants ( IF 4.658 ) Pub Date : 2021-01-17 , DOI: 10.3390/plants10010168
Zafar A. Handoo , Guiping Yan , Mihail R. Kantor , Danqiong Huang , Intiaz A. Chowdhury , Addison Plaisance , Gary R. Bauchan , Joseph D. Mowery

Root-lesion nematodes (Pratylenchus spp.) of the genus Pratylenchus Filipjev, 1936, are among the most important nematode pests on soybean (Glycine max (L.) Merr.), along with soybean cyst and root-knot nematodes. In May 2015 and 2016, a total of six soil samples were collected from a soybean field in Walcott, Richland County, ND and submitted to the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), USDA, ARS, MD for analysis. Later, in 2019, additional nematodes recovered from a greenhouse culture on soybean originally from the same field were submitted for further analysis. Males, females, and juveniles of Pratylenchus sp. were recovered from soil and root samples and were examined morphologically and molecularly. DNA from single nematodes were extracted, and the nucleotides feature of three genomic regions targeting on the D2–D3 region of 28S rDNA and ITS rDNA and mitochondrial cytochrome oxidase subunit I (COX1) gene were characterized. Phylogeny trees were constructed to ascertain the relationships with other Pratylenchus spp., and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to provide a rapid and reliable differentiation from other common Pratylenchus spp. Molecular features indicated that it is a new, unnamed Pratylenchus sp. that is different from morphologically closely related Pratylenchus spp., including P. convallariae, P. pratensis, P. fallax, and P. flakkensis. In conclusion, both morphological and molecular observations indicate that the North Dakota isolate on soybean represents a new root-lesion nematode species which is named and described herein as Pratylenchus dakotaensis n. sp.

中文翻译:

拟南芥的形态和分子特征 sp。(线虫:Pratylenchidae),一种在美国北达科他州的大豆上的新根瘤线虫种

根腐线虫(短体线虫属种)短体线虫Filipjev,1936年,是最重要的线虫中的大豆(大豆(L.)大豆- 。),以及大豆胞囊和根结线虫。在2015年5月和2016年5月,总共从北达科他州里奇兰县沃尔科特的一个大豆田中采集了六个土壤样品,并提交给了美国农业部,美国农业部,医学博士的真菌学和线虫病遗传多样性和生物学实验室(MNGDBL)进行分析。后来,在2019年,从原本来自同一田地的大豆的温室栽培中回收的其他线虫被提交进行进一步分析。ty鼠的雄性,雌性和幼体sp。从土壤和根部样品中回收,并进行形态和分子检查。从单个线虫中提取DNA,并针对28S rDNA和ITS rDNA的D2-D3区和线粒体细胞色素氧化酶亚基I(COX1)基因的三个基因组区域的核苷酸特征进行了表征。构造系统树以确认与其他鼠疫菌的关系,并进行聚合酶链反应-限制性片段长度多态性(PCR-RFLP),以与其他常见鼠疫菌快速,可靠地区分。分子特征表明它是一种新的未命名的Pratylenchus sp。与形态上密切相关的不同短体线虫属,包括Pconvallariae草地早熟禾P.蝗,和Pflakkensis。总之,形态学和分子观察都表明大豆上的北达科他州分离株代表了一种新的根病变线虫物种,其在本文中被命名和描述为Pratylenchus dakotaensis n。sp。
更新日期:2021-01-18
down
wechat
bug