Abstract

A new flavonoid named (2S)-7,4′-dimethoxyl-6-(2″,3″-epoxy-3″-methylbutyl)flavanone (1), along with five known compounds (2–6), were isolated from the EtOAc-soluble extract of the stem bark of Maackia amurensis. Their structures were elucidated on the basis of spectroscopic methods. All compounds were evaluated for anti-inflammatory and antioxidant activities in vitro. Among them, compound 5 showed the highest inhibitory activity on NO production in RAW264.7 cells stimulated by LPS with IC₅₀ value of 59.0 ± 1.5 µM. Meanwhile, compounds 1–6 exhibited varying antioxidant activities through DPPH, ABTS free radical-scavenging and FRAP assays.

摘要

分离出一种名为 (2 S )-7,4'-二甲氧基-6-(2",3"-epoxy-3"-甲基丁基)黄烷酮 ( 1 )的新黄酮类化合物( 1 )，以及五种已知化合物 ( 2-6 )来自Maackia amurensis茎皮的 EtOAc 可溶性提取物。它们的结构是在光谱方法的基础上阐明的。在体外评估了所有化合物的抗炎和抗氧化活性。其中，化合物5对LPS刺激的RAW264.7细胞NO产生的抑制活性最高，IC ₅₀值为59.0 ± 1.5 µM。另一方面，化合物1 - 6 通过 DPPH、ABTS 自由基清除和 FRAP 测定显示出不同的抗氧化活性。