Prediction of bacteria transport to riparian shallow groundwater is very important to prevent the pollution of water resources by coliform bacteria. The objective of this study was to investigate the effectiveness of microbially induced calcium carbonate precipitation (MICCP) in reducing bacteria (Escherichia coli and Pseudomonas fluorescens) transport. To perform the MICCP process, air-dried sterile sand was poured into polyvinyl chloride (PVC) cylinders (4.8 × 14.92 cm) and incubated for 3 days in the presence of Sporosarcina pasteurii (~10⁸ Cell g⁻¹sand), urea and CaCl₂ (1.5 M). A pulse (0.1 pore volume (PV)) of bacteria suspension (10⁸ CFU mL⁻¹) was added on top of the sand columns (MICCP treated and control) and leaching experiments were followed at steady-state, saturated flow condition. Culturable bacteria were determined in the leachate and the sand extracts (resident bacteria) by colony count method. The MICCP treatment reduced saturated hydraulic conductivity (K_s) value 3.9 times compared to the control and thereby reduced bacteria leaching. Bioprecipitated calcium carbonate in MICCP columns increased bacteria straining compared to control columns especially in surface layer (0-3 cm). MICCP process increased filtration coefficient (λ_f), and relative adsorption index (S_R), and decreased maximum depth of bacteria transport (Z_max). The average (C_av) and cumulative cell density (C_cum) of both bacteria passed from the MICCP column decreased around 3.9-fold compared to the control. Overall, the MICCP process has potential in bacteria filtration in the porous media reducing the risk of groundwater pollution.

预测细菌向河岸浅层地下水的迁移，对于防止大肠菌对水资源的污染非常重要。这项研究的目的是调查微生物诱导的碳酸钙沉淀（MICCP）在减少细菌（大肠杆菌和荧光假单胞菌）运输中的有效性。为了执行MICCP过程，将风干的无菌沙子倒入聚氯乙烯（PVC）圆筒（4.8×14.92 cm）中，并在巴斯德孢子菌（〜10 ⁸ Cell g ^-1沙子），尿素和CaCl ₂（1.5 M）。细菌悬浮液（10 ⁸ CFU mL ）的脉冲（0.1孔体积（PV））^将-1）添加到砂柱（经MICCP处理和对照）的顶部，并在稳态，饱和流动条件下进行浸出实验。通过菌落计数法确定渗滤液和沙提取物中的可培养细菌（残留细菌）。与对照相比，MICCP处理将饱和水力传导率（K _s）值降低了3.9倍，从而减少了细菌的浸出。与对照柱相比，MICCP柱中的生物沉淀碳酸钙增加了细菌的应变，特别是在表层（0-3厘米）中。MICCP过程增加过滤系数（λ _˚F），和相对吸附指数（小号_{- [R} ），并降低细菌的运输最大深度（ž_最大）。与对照相比，从MICCP柱传递的两种细菌的平均值（C _av）和累积细胞密度（C _cum）降低了约3.9倍。总体而言，MICCP工艺具有在多孔介质中过滤细菌的潜力，从而降低了地下水污染的风险。