The involvement of DNA methylation in the mechanisms of formation of conditioned food aversion memory was studied on Helix lucorum snails. The dynamics of aversion formation in snails injected with DNA methyltransferase inhibitor RG108 did not differ from that in control snails. The memory was retained for more than one month after training following RG108 injection and the duration of memory persistence did not differ from that in control animals. However, the characteristics of memory in control and experimental snails differed significantly. In control snails, injections of glutamate NMDA-receptor antagonist or protein synthesis inhibitor before memory retrieval caused disorders in the memory reconsolidation and development of amnesia 2 days after training. By contrast, injections of these substances before retrieval to snails trained against the background of RG108 treatment caused no memory disorders. We hypothesized that inhibition of DNA methylation processes led to the formation of strong memory, not reactivated after retrieval and not transformed into a labile state sensitive to amnesic agents.

中文翻译：

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在 DNA 甲基转移酶抑制的背景下学习导致形成对重新激活和损害具有抵抗力的记忆

在 Helix lucorum 蜗牛上研究了 DNA 甲基化在条件性食物厌恶记忆形成机制中的参与。注射了 DNA 甲基转移酶抑制剂 RG108 的蜗牛的厌恶形成动力学与对照蜗牛没有区别。注射RG108后，训练后记忆保持1个多月，记忆持续时间与对照动物无差异。然而，对照蜗牛和实验蜗牛的记忆特征显着不同。在对照蜗牛中，在记忆恢复前注射谷氨酸 NMDA 受体拮抗剂或蛋白质合成抑制剂会导致记忆再巩固障碍和训练后 2 天失忆症的发展。相比之下，在将这些物质回收到在 RG108 治疗背景下训练的蜗牛之前注射这些物质不会导致记忆障碍。我们假设 DNA 甲基化过程的抑制导致强烈记忆的形成，在检索后不会重新激活，也不会转化为对遗忘剂敏感的不稳定状态。