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Leaf-derived ABA regulates rice seed development via a transporter-mediated and temperature-sensitive mechanism
Science Advances ( IF 13.6 ) Pub Date : 2021-01-15 , DOI: 10.1126/sciadv.abc8873
Peng Qin 1 , Guohua Zhang 1 , Binhua Hu 1 , Jie Wu 2 , Weilan Chen 1 , Zhijie Ren 3 , Yulan Liu 1 , Jing Xie 1 , Hua Yuan 1 , Bin Tu 1 , Bingtian Ma 1 , Yuping Wang 1 , Limin Ye 4 , Legong Li 3 , Chengbin Xiang 2 , Shigui Li 1
Affiliation  

Long-distance transport of the phytohormone abscisic acid (ABA) has been studied for ~50 years, yet its mechanistic basis and biological significance remain very poorly understood. Here, we show that leaf-derived ABA controls rice seed development in a temperature-dependent manner and is regulated by defective grain-filling 1 (DG1), a multidrug and toxic compound extrusion transporter that effluxes ABA at nodes and rachilla. Specifically, ABA is biosynthesized in both WT and dg1 leaves, but only WT caryopses accumulate leaf-derived ABA. Our demonstration that leaf-derived ABA activates starch synthesis genes explains the incompletely filled and floury seed phenotypes in dg1. Both the DG1-mediated long-distance ABA transport efficiency and grain-filling phenotypes are temperature sensitive. Moreover, we extended these mechanistic insights to other cereals by observing similar grain-filling defects in a maize DG1 ortholog mutant. Our study demonstrates that rice uses a leaf-to-caryopsis ABA transport–based mechanism to ensure normal seed development in response to variable temperatures.



中文翻译:

叶源ABA通过转运蛋白介导和温度敏感机制调节水稻种子发育

已经研究了植物激素脱落酸 (ABA) 的长距离运输约 50 年,但对其机理基础和生物学意义仍然知之甚少。在这里,我们展示了叶片衍生的 ABA 以温度依赖性方式控制水稻种子的发育,并受到缺陷型灌浆 1 (DG1) 的调节,DG1 是一种多药和有毒的复合挤压转运蛋白,可在节和穗轴处排出 ABA。具体来说,ABA 在 WT 和dg1叶中都被生物合成,但只有 WT 颖果积累叶衍生的 ABA。我们证明叶衍生的 ABA 激活淀粉合成基因解释了dg1中不完全填充和粉状种子表型. DG1 介导的长距离 ABA 运输效率和灌浆表型都是温度敏感的。此外,我们通过观察玉米 DG1 直系同源突变体中类似的籽粒灌浆缺陷,将这些机制见解扩展到其他谷物。我们的研究表明,水稻使用基于叶到颖果 ABA 转运的机制来确保种子正常发育以应对变化的温度。

更新日期:2021-01-15
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