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Expression pattern analysis of three R2R3-MYB transcription factors for the production of anthocyanin in different vegetative stages of Arabidopsis leaves
Applied Biological Chemistry ( IF 3.2 ) Pub Date : 2021-01-15 , DOI: 10.1186/s13765-020-00584-0
Yeonjong Koo , R. Scott Poethig

Anthocyanin is a type of flavonoid that appears purple in plants. PAP1, PAP2, and MYB113 are the three major R2R3-MYB transcription factors that regulate flavonoid biosynthesis in Arabidopsis thaliana. In this study, we found that the three MYB genes regulate anthocyanin accumulation in different leaf stages. Under limited nutrient conditions, PAP1 and PAP2 genes were highly induced in juvenile leaves. Conversely, MYB113 was expressed mainly in adult leaves. In addition, we investigated the role of trans-acting siRNA4 (TAS4) in the post-transcriptional regulation of anthocyanin expression in Arabidopsis leaves. In plant growth, the inhibition of PAP1 and PAP2 gene expression by TAS4 was observed only in juvenile leaves, and MYB113 inhibition was observed in adult leaves. In conclusion, we found that transcription and transcript repression of the three MYB genes is differentially regulated by TAS4 in leaf developmental stages. Our results improve the understanding of the regulation of plant anthocyanin production under stress conditions.

中文翻译:

三种R2R3-MYB转录因子在拟南芥叶片不同营养阶段生产花色苷的表达模式分析

花青素是一种类黄酮,在植物中呈紫色。PAP1,PAP2和MYB113是调节拟南芥中黄酮类生物合成的三个主要R2R3-MYB转录因子。在这项研究中,我们发现三个MYB基因调节花青素在不同叶期的积累。在有限的营养条件下,幼叶中高度诱导了PAP1和PAP2基因。相反,MYB113主要在成年叶片中表达。此外,我们调查了反式siRNA4(TAS4)在拟南芥叶片中花色苷表达的转录后调控中的作用。在植物生长中,仅在少年叶片中观察到了TAS4对PAP1和PAP2基因表达的抑制,而在成年叶片中观察到了MYB113抑制。结论,我们发现,三个MYB基因的转录和转录抑制在叶片发育阶段受TAS4差异调节。我们的结果提高了对胁迫条件下植物花色苷生产调控的认识。
更新日期:2021-01-16
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