This study aimed to investigate the rhizosphere bacterial isolates’ antagonistic property against the soil-borne fungal phytopathogen, Sclerotium rolfsii Sacc. (Athelia rolfsii (Curzi) Tu & Kimbrough). The chemical control of the disease caused by S. rolfsii is economically and environmentally unsustainable, and therefore, a bio-control agent in the form of rhizospheric bacteria is gaining importance. Five rhizospheric Bacillus species viz. B. subtilis subsp. Subtilis str.168 (accession no. MH283878), B. siamensis strain PDA 10 (accession no. MH283879), B. amyloliquefaciens strain 1034 (accession no. MH283880), B. velezensis strain FZB42165 (accession no. MH283881), and B. atrophaeus strain NBRC 15539 (accession no. MH283882) were assessed for their antagonistic potential against S. rolfsii based on 3 different screening methods. Among these, 100% fungal growth inhibition by all 5 Bacillus spp. was observed in the novel ring method, whereas in the dual culture method, the maximum growth inhibition was (58%) exhibited by the strain NBRC 15539 of B. atrophaeus. The antagonistic activity showed by the modified dual culture method was also relatively high, and the highest activity (93.7%) was shown by the strain NBRC 15539 of B. atrophaeus. Besides, the Bacillus sp. was also evaluated for their plant growth-promoting attributes and other properties such as the production of siderophore, HCN, amylase, protease, lipase, and ammonia, including their assessment for chitinase and cellulase activity. The study provided empirical evidence of Bacillus sp. antagonistic potential against S. rolfsii and should be of contributive value in developing a biocontrol agent for this highly important crop fungal pathogen.

中文翻译：

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芽孢杆菌抗菌潜力的评估。对抗土壤传播的真菌病原菌Sclerotium rolfsii Sacc。（Athelia rolfsii（Curzi）Tu和Kimbrough）

这项研究旨在调查根际细菌分离株对土壤传播的真菌植物病原菌，菌核菌Saccrotium rolfsii Sacc的拮抗特性。（Athelia rolfsii（Curzi）Tu和Kimbrough）。由罗氏链球菌引起的疾病的化学控制在经济和环境上都是不可持续的，因此，根际细菌形式的生物控制剂变得越来越重要。五个根际芽孢杆菌种。枯草芽孢杆菌亚种 枯草芽孢杆菌str.168（登录号MH283878），B。siamensis菌株PDA 10（登录号MH283879），解淀粉芽孢杆菌1034（登录号MH283880），B。velezensis菌株FZB42165（登录号MH283881）和B基于3种不同的筛选方法，评估了萎缩杆菌NBRC 15539（登录号MH283882）对罗非鱼的拮抗潜力。在这些当中，所有5个芽孢杆菌属菌种均100％抑制真菌生长。在新型环法中观察到了这种现象，而在双重培养法中，萎缩芽孢杆菌的NBRC 15539菌株表现出最大的生长抑制作用（58％）。改良的双重培养方法显示的拮抗活性也较高，而萎缩双歧杆菌的NBRC 15539菌株表现出最高的拮抗活性（93.7％）。此外，芽孢杆菌属。还评估了其对植物生长的促进作用和其他特性，例如铁载体，HCN，淀粉酶，蛋白酶，脂肪酶和氨的产生，包括对几丁质酶和纤维素酶活性的评估。该研究提供了芽孢杆菌的经验证据。对S有拮抗作用。