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Enhancing and Mitigating Radiolytic Damage to Soft Matter in Aqueous Phase Liquid-Cell Transmission Electron Microscopy in the Presence of Gold Nanoparticle Sensitizers or Isopropanol Scavengers
Nano Letters ( IF 10.8 ) Pub Date : 2021-01-15 , DOI: 10.1021/acs.nanolett.0c04636
Joanna Korpanty 1 , Lucas R. Parent 2 , Nathan C. Gianneschi 1, 3
Affiliation  

In this work, we describe the radiolytic environment experienced by a polymer in water during liquid-cell transmission electron microscopy (LCTEM). We examined the radiolytic environment of aqueous solutions of poly(ethylene glycol) (PEG, 2400 g/mol) in the presence of sensitizing gold nanoparticles (GNPs, 100 nm) or radical scavenging isopropanol (IPA). To quantify polymer damage, we employed post-mortem analysis via matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). This approach confirms IPA (1–10% w/v) can significantly mitigate radiolysis-induced damage to polymers in water, while GNPs significantly enhance damage. We couple LCTEM experiments with simulations to provide a generalizable strategy for assessing radiolysis mitigation or enhancement. This study highlights the caution required for LCTEM experiments on inorganic nanoparticles where solution phase properties of surrounding organic materials or the solvent itself are under investigation. Furthermore, we anticipate an increased use of scavengers for LCTEM studies of all kinds.

中文翻译:

在金纳米粒子增感剂或异丙醇清除剂存在下,增强和减轻水相液体细胞透射电子显微镜中对软物质的放射性损伤

在这项工作中,我们描述了液体细胞透射电子显微镜(LCTEM)期间,聚合物在水中经历的辐射分解环境。我们在敏化金纳米颗粒(GNPs,100 nm)或自由基清除异丙醇(IPA)存在下检查了聚乙二醇(PEG,2400 g / mol)水溶液的辐射环境。为了量化聚合物损坏,我们采用了事后检验通过基质辅助激光解吸/电离成像质谱(MALDI-IMS)分析。该方法证实IPA(1-10%w / v)可以显着减轻辐射分解对水中聚合物的损害,而GNP则可以显着增强损害。我们将LCTEM实验与模拟相结合,以提供一种评估辐射分解缓解或增强的通用策略。这项研究强调了对正在研究周围有机材料或溶剂本身的溶液相性质的无机纳米粒子进行LCTEM实验所需的谨慎性。此外,我们预计清除剂在各种LCTEM研究中的使用将有所增加。
更新日期:2021-01-27
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