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Generation of Nano-pores in Silk Fibroin Films Using Silk Nanoparticles for Full-Thickness Wound Healing
Biomacromolecules ( IF 6.2 ) Pub Date : 2021-01-15 , DOI: 10.1021/acs.biomac.0c01411
Jian Liu 1 , Ran Huang 2 , Gang Li 1 , David L Kaplan 3 , Zhaozhu Zheng 1 , Xiaoqin Wang 1
Affiliation  

Silk fibroin films are used in tissue engineering due to their biocompatibility, optical clarity, and slow biodegradability. However, the relatively smooth surface and low permeability of these systems may limit some applications; thus, here, a method was developed to generate nano-pores in methanol or ethanol-treated silk fibroin films. The first step was to induce the formation of nanoparticles (50–300 nm diam.) in silk fibroin solutions by autoclaving. After drying in air, the films formed were treated to induce silk β-sheet structures, which condense the bulk silk phase and nanoparticles and phase separation and enlarge the space of bulk silk phase and nanoparticles. These films were then extracted with water to allow the condensed nanoparticles to escape, leaving homogeneous nano-pores (50–300 nm) in the silk fibroin matrix. The introduction of nano-pores resulted in enhanced permeability and minimized loss of the mechanical properties of the nano-porous silk fibroin films (NSFs) when compared to the un-autoclaving-treated silk fibroin films. NSFs promoted cell (human fibroblasts) proliferation and oxygen/nutrition perfusion and significantly enhanced the complete skin-thickness wound healing in a rat model, suggesting the potential use in tissue regeneration or as wound dressing biomaterials for clinical applications.

中文翻译:

使用丝纳米粒子用于全层伤口愈合的丝素蛋白膜中的纳米孔的生成。

丝素蛋白薄膜由于其生物相容性,光学透明度和缓慢的生物降解性而用于组织工程。但是,这些系统的相对光滑的表面和低渗透性可能会限制某些应用。因此,在这里,开发了一种在甲醇或乙醇处理的丝素蛋白膜中产生纳米孔的方法。第一步是通过高压灭菌法在丝素蛋白溶液中诱导纳米颗粒(直径50-300 nm)的形成。在空气中干燥后,处理形成的膜以诱导丝β-片层结构,其缩合本体丝相和纳米颗粒以及相分离,并扩大本体丝相和纳米颗粒的空间。然后用水提取这些薄膜,以使凝聚的纳米颗粒逸出,在丝素蛋白基质中留下均匀的纳米孔(50-300 nm)。与未经高压灭菌处理的丝素蛋白薄膜相比,纳米孔的引入可提高渗透性,并使纳米多孔丝素蛋白薄膜(NSFs)的机械性能损失降至最低。NSF促进了细胞(人类成纤维细胞)增殖和氧/营养灌注,并显着增强了大鼠模型中完整的皮肤厚度伤口愈合,表明其可用于组织再生或作为临床应用的伤口包扎生物材料。
更新日期:2021-02-08
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