Abstract

Separation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC by using dimethyl sulfoxide as cosolvent. Ethyl acetate/n-butyl alcohol/0.3% glacial acetic acid (4: 1: 5, v/v) system was used and three target compounds with purity higher than 80% were obtained. Preparative HPLC was used for further purification and three target compounds with purity higher than 98% were obtained. The compounds were identified as chlorogenic acid, pedaliin and pedaliin-6″-acetate.

中文翻译：

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以二甲基亚砜为助溶剂，从唐Tang草粗提物中HSCCC分离三种主要化合物

摘要

直接从粗提物中分离天然化合物是传统柱色谱法的一项艰巨工作。在本研究中，已通过高速逆流色谱法（HSCCC）成功建立了一种从唐古德Dracocephalum tanguticum粗提物中分离三种主要化合物的有效方法。通过使用二甲基亚砜作为助溶剂将粗提取物直接引入HSCCC。使用乙酸乙酯/正丁醇/0.3%冰醋酸（4：1：5，v / v）系统，获得了纯度高于80％的三种目标化合物。用制备型HPLC进一步纯化，得到三种纯度高于98％的目标化合物。化合物被鉴定为绿原酸，脚踏素和脚踏素-6″-乙酸盐。