Eclipta prostrata (L.) is an important and well-known medicinal plant due to its valuable bioactive compounds. Microorganisms, including arbuscular mycorrhizal fungi (AMF), and salinity could directly impact plant metabolome, thus influencing their secondary metabolites and the efficacy of herbal medicine. In this study, the role of different single AMF species (Funneliformis mosseae, Septoglomus deserticola, Acaulospora lacunosa) and a mixture of six AMF species in plant growth and physio-biochemical characteristics of E. prostrata under non-saline conditions was investigated. Next, the most suitable AM treatment was chosen to examine the impact of AMF on physio-biochemical features and polyphenol profiles of E. prostrata under saline conditions (100 and 200 mM NaCl). The findings indicated that AMF mixture application resulted in more effective promotion on the aboveground part of non-saline plants than single AMF species. AM mixture application improved growth and salt tolerance of E. prostrata through increasing the activity of catalase, peroxidase (at 4 weeks), proline, and total phenolic content (at 8 weeks). Such benefits were not observed under high salinity, except for a higher total phenolic concentration in mycorrhizal plants at 8 weeks. Through high-performance liquid chromatography, 14 individual phenolic compounds were analyzed, with wedelolactone and/or 4,5-dicaffeoylquinic acid abundant in all treatments. Salinity and mycorrhizal inoculation sharply altered the polyphenol profiles of E. prostrata. Moderate salinity boosted phenolic compound production in non-AM plants at 4 weeks, while at 8 weeks, the decline in the content of phenolic compounds occurred in uncolonized plants subjected to both saline conditions. Mycorrhization augmented polyphenol concentration and yield under non-saline and saline conditions, depending on the growth stages and salt stress severity. Plant age influenced polyphenol profiles with usually a higher content of phenolic compounds in older plants and changed the production of individual polyphenols of both non-AM and AM plants under non-stress and salt stress conditions. A better understanding of factors (involving mycorrhiza and salinity) affecting the phenolic compounds of E. prostrata facilitates the optimization of individual polyphenol production in this medicinal plant.

旱莲草(L.) 因其有价值的生物活性化合物而成为一种重要且众所周知的药用植物。包括丛枝菌根真菌（AMF）在内的微生物和盐度可以直接影响植物的代谢组，从而影响其次生代谢产物和草药的功效。在本研究中，不同单一 AMF 物种的作用（苔藓漏斗形虫,沙漠七球藻,空隙无梗孢菌）和六种 AMF 的混合物对植物生长和生理生化特性的影响匍匐桉在非盐条件下进行了研究。接下来，选择最合适的AM处理来检查AMF对生理生化特征和多酚谱的影响匍匐桉在盐水条件下（100 和 200 mM NaCl）。研究结果表明，AMF 混合物的施用比单一 AMF 物种对非盐生植物的地上部分产生更有效的促进作用。AM混合物的施用改善了生长和耐盐性匍匐桉通过增加过氧化氢酶、过氧化物酶（4 周时）、脯氨酸和总酚含量（8 周时）的活性。除了8周时菌根植物中总酚浓度较高之外，在高盐度下没有观察到这种益处。通过高效液相色谱法，分析了 14 种酚类化合物，其中所有处理中都富含蟛蜞菊内酯和/或 4,5-二咖啡酰奎宁酸。盐度和菌根接种急剧改变了多酚谱匍匐桉。中等盐度在第 4 周时提高了非 AM 植物中酚类化合物的产量，而在第 8 周时，在两种盐分条件下的未定殖植物中酚类化合物的含量出现下降。在非盐和盐条件下，菌根增加了多酚浓度和产量，具体取决于生长阶段和盐胁迫严重程度。植物年龄影响多酚谱，老植物中酚类化合物的含量通常较高，并改变非 AM 和 AM 植物在非胁迫和盐胁迫条件下的单个多酚的产量。更好地了解影响酚类化合物的因素（涉及菌根和盐度）匍匐桉有助于优化这种药用植物中单个多酚的生产。