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RNA Interference by Cyanobacterial Feeding Demonstrates the SCSG1 Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in Stentor
Microorganisms ( IF 4.5 ) Pub Date : 2021-01-15 , DOI: 10.3390/microorganisms9010176
Wei Wei , Chuanqi Jiang , Xiaocui Chai , Juyuan Zhang , Cheng-Cai Zhang , Wei Miao , Jie Xiong

In the giant ciliate Stentor coeruleus, oral apparatus (OA) regeneration is an experimentally tractable regeneration paradigm that occurs via a series of morphological steps. OA regeneration is thought to be driven by a complex regulatory system that orchestrates the temporal expression of conserved and specific genes. We previously identified a S. coeruleus-specific gene (named SCSG1) that was significantly upregulated during the ciliogenesis stages of OA regeneration, with an expression peak at the stage of the first OA cilia appearance. We established a novel RNA interference (RNAi) method through cyanobacteria Synechocystis sp. PCC6803 feeding in S. coeruleus. The expression of SCSG1 gene was significantly knocked down by using this method and induced abnormal ciliogenesis of OA regeneration in S. coeruleus, suggesting that SCSG1 is essential for OA regeneration in S. coeruleus. This novel RNAi method by cyanobacterial feeding has potential utility for studying other ciliates.

中文翻译:

蓝藻摄食的RNA干扰表明SCSG1基因对于Stentor口腔器械再生过程中的睫状发生至关重要。

在巨大的纤毛Stentor蓝藻中,口腔器皿(OA)再生是一种通过一系列形态学步骤发生的实验上易处理的再生范例。OA再生被认为是由一个复杂的调控系统驱动的,该调控系统可以协调保守和特定基因的时间表达。我们先前识别的一个S.斑特异性基因(命名单元SCSG1期间OA再生ciliogenesis阶段被显著上调,与在第一OA纤毛外观的阶段的表达峰)。我们通过蓝藻集胞藻sp。建立了一种新型的RNA干扰(RNAi)方法。PCC6803饲喂蓝藻SCSG1的表达基因显著撞倒通过使用该方法和在OA再生的诱导异常ciliogenesis S.斑,这表明单元SCSG1是用于OA再生必不可少S.斑。这种通过蓝细菌饲养的新颖RNAi方法在研究其他纤毛虫中具有潜在的实用性。
更新日期:2021-01-15
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