The antioxidative activity of Camelia sinensis tea and especially powdered tea extracts on the market, among others used as added value in functional foods, can considerably vary due to not only natural variance, but also adulteration and falsification. Thus, an effect-directed profiling was developed to prove the functional effects or health-promoting claims. It took 3–12 min per sample, depending on the assay incubation time, for 21 separations in parallel. Used as a fast product quality control, it can detect known and unknown bioactive compounds. Twenty tea extracts and a reference mixture of 11-bioactive compounds were investigated in parallel under the same chromatographic conditions by a newly developed reversed phase high-performance thin-layer chromatographic method. In eight planar on-surface assays, effect-directed tea profiles were revealed. Catechins and theaflavins turned out to be not only highly active, but also multi-potent compounds, able to act in a broad range of metabolic pathways. The flavan-3-ols acted as radical scavengers (DPPH^· assay), antibacterials against Gram-positive Bacillus subtilis bacteria, and inhibitors of tyrosinase, α-glucosidase, β-glucosidase, and acetylcholinesterase. Further effects against Gram-negative Aliivibrio fischeri bacteria and β-glucuronidase were assigned to other components in the powdered tea extracts. According to their specifications, the activity responses of the powdered tea extracts were higher than in mere leaf extracts of green, white and black tea. The multi-imaging and effect-directed profiling was not only able to identify known functional food ingredients, but also to detect unknown bioactive compounds (including bioactive contaminants, residues or adulterations).

中文翻译：

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儿茶素，茶黄素，黄酮醇和咖啡因的茶粉提取物的效果导向分析

茶花的抗氧化活性茶叶，尤其是市场上的茶粉提取物，尤其是在功能性食品中用作附加值，可能不仅由于自然差异，而且由于掺假和伪造而有很大差异。因此，开发了以效果为导向的配置文件，以证明功能效果或促进健康的主张。每个样品需要3–12分钟，具体取决于测定的孵育时间，以平行进行21次分离。用作快速产品质量控制，它可以检测已知和未知的生物活性化合物。通过新开发的反相高效薄层色谱法在相同的色谱条件下平行研究了二十种茶提取物和11种生物活性化合物的参考混合物。在八种平面表面分析中，揭示了效果导向的茶叶轮廓。儿茶素和茶黄素不仅具有很高的活性，而且具有多种有效的化合物，能够在广泛的代谢途径中起作用。黄烷-3-醇充当自由基清除剂（DPPH^·测定），针对革兰氏阳性枯草芽孢杆菌细菌的抗菌剂以及酪氨酸酶，α-葡萄糖苷酶，β-葡萄糖苷酶和乙酰胆碱酯酶的抑制剂。茶粉提取物中的其他成分还具有针对革兰氏阴性阿氏弧菌细菌和β-葡萄糖醛酸苷酶的进一步作用。根据它们的规格，茶粉提取物的活性响应高于绿茶，白茶和红茶的纯叶提取物。多重成像和效果导向的配置文件不仅能够识别已知的功能性食品成分，而且还能够检测未知的生物活性化合物（包括生物活性污染物，残留物或掺假物）。