The colorectal cancer is a serious health problem. The diagnosis of the disease mostly relies on an invasive procedure. A non-invasive diagnostic test such as an immunoassay, may facilitate diagnosis of colorectal cancer. The purpose of the study was to evaluate the use of antibodies against Fusobacterium nucleatum in the diagnosis of colorectal cancer (CRC). Totally 78 patients in three groups were included in the study. F. nucleatum in the tissues was detected using quantitative polymerase chain reaction assay. F. nucleatum IgA and IgG were measured using enzyme linked immunosorbent assay. F. nucleatum was detected in 86.7% and 73.1% cases of CRC and precancerous-benign colon disease (P-BCD), respectively. The OD values from F. nucleatum IgA and IgG ELISA tests were higher in CRC group compared with healthy individuals. The sensitivity of IgA ELISA test varied between 31.8 and 95.5% depending on the chosen cut-off values. The positivity rate of antibodies in patients with high amount of F. nucleatum in tissue was significantly greater than in the negative group. The F. nucleatum IgA and IgG antibodies in CRC were higher than the ones in healthy controls but the discriminative ability of the ELISA test was not adequate to be considered as a diagnostic tool.

结直肠癌是一个严重的健康问题。该疾病的诊断主要依赖于侵入性程序。非侵入性诊断测试，如免疫测定，可能有助于诊断结直肠癌。本研究的目的是评估具核梭杆菌抗体在结直肠癌 (CRC) 诊断中的应用。三组共78例患者纳入研究。使用定量聚合酶链反应测定法检测组织中的具核梭杆菌。F. nucleatum IgA 和 IgG 使用酶联免疫吸附试验测量。F. nucleatum分别在 86.7% 和 73.1% 的 CRC 和癌前良性结肠疾病 (P-BCD) 病例中检测到。OD 值来自与健康个体相比，CRC 组有核梭杆菌IgA 和 IgG ELISA 检测较高。IgA ELISA 测试的灵敏度在 31.8% 到 95.5% 之间变化，具体取决于所选的临界值。组织中具核梭菌含量高的患者抗体阳性率明显高于阴性组。该具核在CRC IgA和IgG抗体在健康对照组比那些较高，但ELISA试验的甄别能力是不足以被视为一种诊断工具。