Abstract

The aim of this study was to examine the effect of different levels of Lactobacillus species and yeast (Saccharomyces cerevisiae) as a toxin binder in deoxynivalenol (DON) diet on immune parameters, gut morphology and jejunal gene expression in broiler chickens. Three hundred sixty-one-day-old female broilers were assigned into nine treatments with four replicates each in a completely randomised design (3 × 3 factorial arrangement) including three levels of Lactobacillus (0, 0.20 and 0.40 g/kg) and yeast (0, 0.75 and 1.50 g/kg) in DON contaminated diets. The results showed that DON challenged birds showed suppressed spleen relative weight and reduced white blood cell and lymphocyte percentage, while inclusion the highest level of Lactobacillus sp. and yeast to DON diet improved these parameters (p < .05). Newcastle antibody titre was increased (p < .05) by increasing the level of Lactobacillus sp. and yeast in DON included diet. Increasing the level of Lactobacillus sp. and yeast in DON diet was linearly enhanced (p < .05) the villus height, muscular layer and absorptive surface area in ileum. Combination of 0.40 g/kg Lactobacillus sp. and 1.50 g/kg yeast caused an enhancement (p < .05) in mucosa and muscular layer thicknesses of jejunum and ileum, respectively. The expressions of toll like receptor-4 and claudin-5 were down-regulated and up-regulated respectively by administration of the highest level of Lactobacillus sp. and yeast. In conclusion, the results indicated that dietary inclusion of the highest level of Lactobacillus sp. and yeast improved the spleen weight, some immunological parameters, villus height, muscular layer thickness and gene expressions in DON- challenged broilers.

• HIGHLIGHTS

• Deoxynivalenol (DON) consumption (10 mg/kg) induce intestinal and immunological changes in broilers.

• Combination of Lactobacillus sp. and yeast reduce DON toxicity.

• Inclusion 0.4 g/kg lactobacillus and 1.5 g/kg yeast improve villus height and humoral immunity.

摘要

这项研究的目的是检验不同水平的乳酸杆菌和酵母（酿酒酵母）作为脱氧雪腐烯醇（DON）饮食中的毒素结合剂对肉鸡免疫参数，肠道形态和空肠基因表达的影响。将361天大的雌性肉鸡分为九种处理方法，每组一式四份，采用完全随机设计（3×3因子排列），包括三种水平的乳杆菌（0、0.20和0.40 g / kg）和酵母菌（ 0、0.75和1.50 g / kg）。结果表明，受DON攻击的鸟类显示出脾脏相对体重降低，白细胞和淋巴细胞百分比降低，而乳酸菌含量最高sp。酵母和DON饮食改善了这些参数（p  <.05）。新城疫抗体滴度 通过增加乳杆菌sp的水平而增加（p <0.05）。DON中的酵母包括饮食。增加乳酸杆菌的水平。DON饮食中的酵母线性增加（p  <.05）回肠中的绒毛高度，肌肉层和吸收表面积。0.40 g / kg乳酸菌sp。和1.50 g / kg酵母引起的增强（p 空肠和回肠的黏膜和肌肉层厚度分别<0.05）。通过给予最高水平的乳酸杆菌属sp ，toll​​样受体4和claudin-5的表达分别下调和上调。和酵母。总之，结果表明，饮食中乳酸菌的含量最高。酵母可以改善DON肉鸡的脾脏重量，一些免疫学参数，绒毛高度，肌肉层厚度和基因表达。

• 强调

• 食用脱氧雪腐烯酚（DON）（10 mg / kg）会引起肉鸡肠道和免疫学改变。

• 乳杆菌sp。的组合。和酵母会降低DON的毒性。

• 包含0.4 g / kg乳酸菌和1.5 g / kg酵母可提高绒毛高度和体液免疫力。