Blood flow functional imaging is widely applied in biological research to provide vascular morphological and statistical parameters. It relies on the absorption difference and is, therefore, easily affected by complex biological structures, and it cannot accommodate abundant functional information. We propose a full‐field multi‐functional angiography method to classify arteriovenous vessels and to display flow velocity and vascular diameter distribution simultaneously. Unlike previous methods, an under‐sampled laser Doppler acquisition mode is used to record the low‐coherence speckle, and multi‐functional angiography is achieved by modulating the endogenous hemodynamic characteristics from low‐coherence speckle. To demonstrate the combination of classified angiography, blood flow velocity measurement, and vascular diameter measurement realized using our method, we performed experiments on the flow phantom and living chicken embryos and generated multi‐functional angiograms. The proposed method can be used as a label‐free multi‐functional angiography technique in which red blood cells provide a strong endogenous source of naturally hemodynamic characteristics.

中文翻译：

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基于内源性血流动力学特征的全视野光学多功能血管造影

血流功能成像已广泛应用于生物学研究中，以提供血管形态学和统计参数。它依赖于吸收差异，因此容易受到复杂的生物结构的影响，并且无法容纳丰富的功能信息。我们提出了一种全视野多功能血管造影方法，以对动静脉血管进行分类，并同时显示流速和血管直径分布。与以前的方法不同，欠采样激光多普勒采集模式用于记录低相干斑点，通过调节低相干斑点的内源性血流动力学特征可实现多功能血管造影。为了展示分类血管造影，血流速度测量，并使用我们的方法实现的血管直径测量，我们对流动体模和活鸡胚进行了实验，并生成了多功能血管造影照片。所提出的方法可以用作无标记的多功能血管造影技术，其中红细胞提供了强大的自然血液动力学特征的内源性来源。